Research Article

High-Intensity Interval Training Improves Physical Function, Prevents Muscle Loss, and Modulates Macrophage-Mediated Inflammation in Skeletal Muscle of Cerebral Ischemic Mice

Figure 8

Level of cytokines in paretic skeletal muscle and lymphocyte subsets in peripheral blood. (a) The profiles of multiple cytokines in paretic gastrocnemius muscle in each group, including interleukin- (IL-) 1α, IL-1β, IL-6, IL-10, IL-27, monocyte chemoattractant protein (MCP-1), tumor necrosis factor- (TNF-) α, and interferon- (IFN-) γ. (b) Representative fluorescence activated cell sorting (FACS) plots of F4/80+CD11b+ macrophages gated on CD45+ cells, CD4+ cells gated on CD45+CD3+ cells, and CD8+ cells gated on CD45+CD3+cells in muscle tissue. (c) Bar graph showing the percentage of F4/80+CD11b+ cells in CD45+ cells, CD4+ cells in CD45+CD3+ cells, CD8+ cells in CD45+CD3+ cells, and the ratio of CD4+ and CD8+ cells. Values are expressed as the of the mean. and compared with the sham group; # and ## compared with the MCAO group; + and ++ compared with the MOD group as determined by one-way ANOVA (Tukey’s multiple comparison test) for the data with normal distribution. The letters for no significance were not shown.
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