Cholinergic Elicitation Prevents Ventricular Remodeling via Alleviations of Myocardial Mitochondrial Injury Linked to Inflammation in Ischemia-Induced Chronic Heart Failure Rats

Figure 9

Impacts of TNF-α exposure on mitochondrial function of H9C2 cell lines. Evaluation of cell variability by cell growth curve (a). Screening of the optimal TNF-α-disposed concentration by ROS fluorescence intensity detections (b). Detections of cell proliferation by CCK-8 assay (c). Measurements of apoptosis rate, MMP, and mPTP by flow cytometry (d–f). Results of mitochondrial dynamics-related proteins between control and experimental groups by western blot (g). vs. control group. ROS: reactive oxygen; MMP: mitochondrial membrane potential; mPTP: mitochondrial permeability transition pore.

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