Migration, Distribution, and Safety Evaluation of Specific Phenotypic and Functional Mouse Spleen-Derived Invariant Natural Killer T2 Cells after Adoptive Infusion

Figure 2

Migration path of iNKT cells tracked by caliper IVIS Lumina II. (a–c) Within 120 min after adoptive infusion of DiR-labeled iNKT cells into mice. The migration path of iNKT cells in mice was monitored from the body surface of mice lying in the supine, lateral, and prone positions. (d) Mice were dissected, and organs were isolated and then detected by fluorescence (iNKT cells infused into the caudal vein first reached the lung, then the liver, and finally the spleen. No fluorescence was detected in the thymus or the inguinal lymph nodes: (1) thymus, (2) spleen, (3) liver, (4) inguinal lymph nodes, and (5) lung). (e) The change in the average fluorescence signal intensity in the spleen, liver, and lung.

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