The Severity of CVB3-Induced Myocarditis Can Be Improved by Blocking the Orchestration of NLRP3 and Th17 in Balb/c Mice

Figure 5

Orchestration of NLRP3 inflammasome and Th17 cells in CVB3-induced myocarditis. (a) The relative mRNA expression of IL-1β, IL-18, NLRP3, Caspase-1, and IL-17 in N and N+ATP groups in the coculture system. (b) The proportion of Th17 cells in N and N+ATP groups in the coculture system. (c) Statistic calculation column of Th17 proportion in N and N+ATP groups in the coculture system. (d) The protein level of NLRP3 in N and N+ATP groups in the coculture system. (e) The protein level of Caspase-1 in N and N+ATP groups in the coculture system. (f) The relative mRNA levels of IL-1β, IL-18, NLRP3, and Caspase-1 in the CD11c⁺ DC cells stimulated with or without IL-17. (g) The protein level of NLRP3 in CD11c⁺ DC cells stimulated with or without IL-17. (h) The protein level of Caspase-1 in CD11c⁺ DC cells stimulated with or without IL-17. (a–e) were acquired from the CD11c⁺ DC and CD4⁺ T cell coculture system stimulated by LPS with (N+ATP) or without ATP (N). (f–h) were acquired from CD11c⁺ DC cells stimulated with IL-17 (N+IL-17) or without IL-17 (N). Statistical description: the value in the figure has been marked between the corresponding groups.

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