The Severity of CVB3-Induced Myocarditis Can Be Improved by Blocking the Orchestration of NLRP3 and Th17 in Balb/c Mice

Figure 7

Knockout of NLRP3 significantly alleviates the severity of CVB3-induced VMC in Balb/c. (a) Statistical analysis of survival rate from day zero to day fourteen in WT and NLRP3^-/- group. (b) HE images of myocardial in WT and NLRP3^-/- groups at fourteenth day of CVB3-induced VMC process (original magnification ×100 and ×200). (c) Masson-trichrome staining of myocardial in WT and NLRP3^-/- groups at fourteenth day of CVB3-induced VMC process (original magnification ×100). (d) IHC images of IL-17 in myocardial in WT and NLRP3^-/- groups at fourteenth day of CVB3-induced VMC process (original magnification ×100). (e) IL-17 expression in heart tissue acquired by IHC assay and IOD value was calculated by Image-Pro Plus. (f, g) The relative mRNA expression of IL-17 and RORγt in myocardium in WT and NLRP3^-/- groups at fourteenth day of CVB3-induced VMC process. (h) The protein level of IL-17 in plasma in WT and NLRP3^-/- groups at fourteenth day of CVB3-induced VMC process. (i) The proportion of Th17 cells in spleen samples in WT and NLRP3^-/- groups at fourteenth day of CVB3-induced VMC process. (j) Statistical histogram of Th17 proportion in (i). Statistical description: the value in the figure has been marked between the corresponding groups.

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