p300/Sp1-Mediated High Expression of p16 Promotes Endothelial Progenitor Cell Senescence Leading to the Occurrence of Chronic Obstructive Pulmonary Disease

Figure 4

Increased p300 mediated the high expression of p16. (a) Western Blot was used to detect the expression of p300 in peripheral blood EPCs of nonsmoking non-COPD, smoking non-COPD, and smoking COPD patients setting each group with 20 samples. (b) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE, p300 was knocked down by siRNA, and the expression of p16 was detected by RT-PCR. (c) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE, p300 was knocked down by siRNA, and the expression of p16 was tested by Western Blot. (d) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE and p300 small molecule inhibitor C646, and the expression of p16 was detected by RT-PCR. (e) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with 5% CSE and p300 small molecule inhibitor C646, and the expression of p16 was tested by Western Blot. , , and .

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