Research Article

Ellagic Acid Alleviates Rheumatoid Arthritis in Rats through Inhibiting MTA1/HDAC1-Mediated Nur77 Deacetylation

Figure 6

Nur77 knockdown reversed the effects of EA on proliferation, inflammation, oxidative stress, and apoptosis in MH7A cells. TNF-α-treated MH7A cells were treated with 50 μM EA alone or together with si-Nur77. (a) The mRNA expression of Nur77 was detected by RT-qPCR. (b) The protein expression of Nur77 was detected by Western blot analysis. (c) CCK-8 assay was performed to measure MH7A cell proliferation. (d, e) MH7A cell apoptosis was measured by flow cytometry. (f, g) The levels of IL-6 and IL-1β in cell culture supernatant were detected by ELISA. (h, i) The levels of SOD and MDA in MH7A cells were examined by commercial kits. Data from at least three replicate experiments were presented as . . .
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