Ataxin-10 Inhibits TNF-α-Induced Endothelial Inflammation via Suppressing Interferon Regulatory Factor-1
Effects of Ataxin-10 on chemokine/cytokine expression in TNF-α-stimulated HUVECs. (a) HUVECs were transfected with either EV or HA-Ataxin-10 for 48 h, followed by incubation with TNF-α (10 ng/mL) for 4 h. Relative mRNA levels of IL-1β, IL-6, CCL-5, CCL-7, CXCL-9, CXCL-1, MCP-1, and TNF-α were detected by qPCR, with β-actin as an internal control. and vs. EV. (b) Ataxin-10 was knocked down using siRNA and then stimulated with TNF-α for 4 h. mRNA levels of CCL-5, CXCL-1, MCP-1, and TNF-α were determined by qPCR. and vs. si-control group.
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