Contribution of Thrombospondin-1 and -2 to Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome

Figure 2

Effect of TSP-2 overexpression on LPS-induced apoptosis. MH-S murine alveolar macrophage cells were either untreated (normal and nontreatment) or treated with LPS (1 μg/ml) for 24 h following either empty vector (vehicle group), TSP-1 (TSP-1 group), or TSP-2 (TSP-2 group) overexpression. Cells were stained with caspase-3 (red) antibody to assess apoptosis (a–d), respectively. Hoechst 33342 dye (blue) was used to stain the nucleus. Green stain represents TSP-1 and TSP-2 overexpression. The yellow color depicts overlay image of caspase-3 and TSP expression in the same cell. (e) Bar graph representing overall quantitation and parallel comparison of caspase-3 staining among the four groups. The percentage of labeled cells/total cell numbers are shown as . value of < 0.05 represents significant difference. ^a compared with normal control; ^b compared with the vehicle group; ^c compared with the TSP-1 transfected group ( per group).

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