Sphingosine Kinase 1 Plays an Important Role in Atorvastatin-Mediated Anti-Inflammatory Effect against Acute Lung Injury

Figure 2

Atorvastatin attenuated HT-mediated cytokine generation in BAL, but SphK1 knockout diminished this effect. ELISA was performed on the BAL harvested from both post-HT WT and SphK1^-/- mice as described in Materials and Methods. Similar with protein and cell infiltration, HT significantly increased cytokine release in WT mouse BAL. These cytokines included IL6, KC, MIP-1α, MIP-2, and MCP-1, but not IL-1β. HT-mediated cytokine release in WT mouse BAL was significantly attenuated by pretreatment of atorvastatin; HT-mediated cytokine generation was significantly higher in SphK1^-/- mouse BAL than that in WT mice. Moreover, SphK1 knockout reversed the anti-inflammatory effect of atorvastatin in cytokine generation (, ).

(a)

(b)

(c)

(d)

(e)

(f)