An Aptamer-Based Antagonist against the Receptor for Advanced Glycation End-Products (RAGE) Blocks Development of Colorectal Cancer

Figure 3

Apt-RAGE inhibited proliferation and migration of colorectal cancer cells. (a) Schematic illustration of Apt-RAGE effect on proliferation and migration of cancer cells. (b) Effect of Apt-RAGE (100 nM) on proliferation of HCT116 cells promoted by S100B (2 μg/mL) was detected by CCK8 cell counting kit at 24 h, 48 h, and 72 h. Data are presented as the , p < 0.01 vs. untreated control and ^# vs. S100B. (c) Quantitation of the effect of Apt-RAGE (100 nM) on migration induced by S100B (2 μg/mL) in wound healing assay at 24 h or 48 h. vs. untreated group and ^## vs. S100B-treated group. n.s. indicates that the difference is not significant compared with the S100B-treated group. (d) Quantitative analysis of the effect of Apt-RAGE (100 nM) on directional migration induced by S100B (2 μg/mL) evaluated using Transwell assay. Upper panel: representative image of the membrane with migrated cells, scale bar: 200 μm. Lower panel: analysis of migrated cells using Transwell assay. Data are presented as the , vs. untreated control, ^## vs. S100B-treated group. n.s. indicates that the difference is not significant compared with the S100B-treated group.

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