Exosomes Containing LINC00636 Inhibit MAPK1 through the miR-450a-2-3p Overexpression in Human Pericardial Fluid and Improve Cardiac Fibrosis in Patients with Atrial Fibrillation

Figure 6

miR-450a-2-3p inhibits TGF-β1-induced EMT of HUVECs induced by TGF-β1. miR-450a-2-3p, VE-Cad, CD31, vimentin, COL1, and COL3 mRNA levels in HUVECs transfected with mimics (a)–(f) and inhibitor (g)–(l) were determined via RT-qPCR. The protein levels of VE-Cad, CD31, vimentin, COL1, and COL3 in CFs transfected with mimics (m) and inhibitor (n) were examined using western blot. Expression levels of miRNAs were normalized to that of U6, and the expression levels of mRNAs and proteins were normalized to that of actin. Representative images of VE-Cad immunofluorescence staining in HUVECs transfected with mimics (o) and inhibitor (p) (×200 magnification) showed the antifibrotic effect of miR-450a-2-3p. Representative images of the scratch assay of mimic- (q) and inhibitor-transfected (r) HUVECs (×100 magnification). Data is presented as the of three independent experiments. , , , .

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