The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway

Figure 4

MiR-766-3P targeted S1PR3. (a) Prediction of miR-766-3p by TargetScan, miRBase and miRTarBase. (b) Immunohistochemistry of S1PR3 in normal and psoriasis tissues. (c) Expression level of S1PR3 in psoriasis tissues by qRT-PCR (n =3). (d) Expression level of S1PR3 in miR-766-3p mimics/NC- or inhibitor/NC-induced HaCaT cells by qRT-PCR. (e) The partial binding sites between miR-766-3p and S1PR3 are shown. (f) Luciferase activity was detected in HEK293 cells cotransfected with miR-766-3p mimics/NC and WT and MUT S1PR3. Data were shown as the mean ± SD, P <0.05, P<0.001. All the experiments were repeated at least three times.

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