The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway

Figure 5

The lncRNA H19/miR-766-3p/S1PR3 axis affects cell proliferation and inflammation in psoriasis via the AKT/mTOR pathway. (a-b) Cotransfection of PGMLV-H19 plasmid with miR-766-3p mimics in M5-induced HaCaT cells; expression level of S1PR3 by qRT-PCR (a); and western blot (b). (c) Proliferative effects of lncRNA H19 were assessed by CCK-8. (d) Proliferative effects of cotransfection of PGMLV-H19 plasmid with miR-766-3p mimics were assessed by CCK-8. (e-h) HaCaT cells were transfected with PGMLV-H19/NC (e-f) and cotransfection of PGMLV-H19 plasmid with miR-766-3p mimics (g-h); ELISA showed the expression level of IL-17A (e, g) and IL-22 (f, h). (i) Western blot showed the expression level of p-mTOR, mTOR, AKT, p-AKT in M5-induced HaCaT cells transfected with PGMLV-H19 plasmid and miR-766-3p mimics. (j-l) Cotransfection of miR-766-3p mimics with MK-2206; proliferative effects were assessed by CCK-8 (j); ELISA showed the expression level of IL-17A (k) and IL-22(l). Data were shown as the mean ± SD, P <0.05, P <0.01, P<0.001. All the experiments were repeated at least three times.

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