Therapeutic Potential of Synthetic Human β-Defensin 1 Short Motif Pep-B on Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells

Figure 4

Anti-inflammatory effects of Pep-B in hDPSCs. Cells were treated with LPS (1 μg/ml) in the presence or absence of Pep-B (2.5, 5 μg/ml). (a) qRT-PCR analysis to quantify inflammatory marker gene expression of different groups in hDPSCs. (b) The protein expression of p65, phospho-ERK, phospho-p38, and phospho-JNK was determined by means of western blot analysis. (c) Quantification of the band density observed in (b) determined using ImageJ software. , , ##, represents comparison with the control group, # represents comparison with the LPS group.

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