Research Article

lnc-MRGPRF-6:1 Promotes M1 Polarization of Macrophage and Inflammatory Response through the TLR4-MyD88-MAPK Pathway

Figure 2

Knockout of lnc-MRGPRF-6:1 can reduce the lipid accumulation and ROS levels of foam cells, significantly increase the viability of macrophages, and inhibit cell apoptosis. After the lnc-MRGPRF-6:1 knockout macrophage was induced to differentiate with the control cells, the cells were stimulated with 50 mg/mL ox-LDL for 24 h. (a) The expression level of lnc-MRGPRF-6:1 between KO and control was detected by RT-qPCR. (b) Image of Oil Red O staining. (c) Viability of macrophage. (d) ROS levels in macrophage. (e) Apoptosis level of macrophage. KO vs. control, , , , and . KO: lnc-MRGPRF-6:1 knockout macrophage; control: macrophage infected by control lentivirus.
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