lnc-MRGPRF-6:1 Promotes M1 Polarization of Macrophage and Inflammatory Response through the TLR4-MyD88-MAPK Pathway

Figure 3

lnc-MRGPRF-6:1 was highly expressed in M1 macrophage. (a, b, g) M1 polarization was stimulated by IFN-γ/LPS, and M2 polarization was stimulated by IL-4. The levels of TNF-α, TNF-β, and IL-10 in the supernatant were detected by ELISA, and the mRNA levels of CXCL10, CXCL11, IL-1β, ARG1, and CCL17 were detected by RT-qPCR. (c, h) The expression levels of lnc-MRGPRF-6:1 in macrophages with different phenotypes were detected by RT-qPCR. (d, e, i) After repolarization, CXCL10, CXCL11, IL-1β, ARG1, and CCL17 levels were detected by RT-qPCR, and TNF-α, TNF-β, and IL-10 were detected by ELISA. (f, j) The expression levels of lnc-MRGPRF-6:1 in repolarization macrophages were detected by RT-qPCR. (a–c, g, h) M1/M2 vs. M0, ns: not statistically significant; , , , and ; M1 vs. M2, ^###; (d–f, i, j) M2-M1/M1-M2 vs. M0, ns: not statistically significant; , , , and ; M2-M1 vs. M1-M2, ^##.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)