CD4⁺ T Cells Promote IgG Production in MHC-Independent and ICAM-1-Dependent Manners in Pristane-Induced Lupus Mice

Figure 7

ICAM-1 blockade leads to the decrease in Treg in the coculture of CD4⁺ T cells and B cells. CD4⁺ T cells and B cells were sorted from PBS and pristane-treated BALB/c mouse (6 months later) by MACS, respectively. CD4⁺ T cells originated from PBS or pristane-treated mice were cultured with B cells derived from PBS mice (on a BALB/c background). After coculturing for 8 days, Foxp3⁺CD4⁺ Treg cells were determined by flow cytometer in the coculture of CD4⁺ T cells and B cells from the PBS group (a–c) and that from the pristane-treated group (d–f). (g–i) The proportions and numbers of Treg in activated CD4⁺ T-B coculture upon ICAM-1 blockade were determined by flow cytometer. ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)