Attenuation of Inflammatory Responses in Breast and Ovarian Cancer Cells by a Novel Chalcone Derivative and Its Increased Potency by Curcumin

Figure 3

The DNA-binding activity of NF-κB in LPS-stimulated (a) MDA-MB-231 cells and (b) SKOV3 cells, in response to various concentrations of MPP with or without 10 μM curcumin. The error bar represents the standard deviation (SD) of mean from three separate experiments. ^### compared with untreated unstimulated control cells; and compared with LPS-stimulated untreated cells. The results of docking experiment, representing NF-κB1 active site containing (c) MPP and (d) curcumin. (e) Superimposition of MPP (cyan) on curcumin (gray) in NF-κB1 active site. The levels of IκB in (f) MDA-MB-231 cells and (g) SKOV3 cells, normalized to β-actin levels, performed by Western blotting.

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