Research Article

Generation of Multispot PSF for Scanning Structured Illumination via Phase Retrieval

Figure 5

The Fourier domain representation of the illumination pattern obtained by the multispot scanning SIM method. The conventional SIM illumination patterns would only contain the peaks at frequencies and 0. Here, the spectral leakage is noted by the red ovals. In principle, the phase retrieval algorithm could produce a multispot PSF whose sinusoidal scanning pattern is arbitrarily close to the ideal sinusoid.