Additive In Vitro Antiplasmodial Effect of N-Alkyl and N-Benzyl-1,10-Phenanthroline Derivatives and Cysteine Protease Inhibitor E64

Figure 2

Layout of a combination experiment in a 96-well plate with the concentration ratios of drug A and drug B prepared as six solutions. When plates were prepared as described in the text, clear wells serve as aquadest, dot black wells serve as a parasite control (no drug, 0% growth inhibition), and wells labeled 1 to 6 serve as drug wells for six drug combination solution, in triplicate, with the wells in row F holding the lowest drug concentration. Both 96-well plates were prepared similarly, with row F₂ representing solutions 4 to 6 in the second 96-well plate. Drug A was (1)-N-alkyl and (1)-N-benzyl-1,10-phenanthroline derivatives, drug B was either E64 or chloroquine.