Acetazolamide Treatment Prevents Redistribution of Astrocyte Aquaporin 4 after Murine Traumatic Brain Injury
Acetazolamide reduced cytotoxic edema in cell bodies within the cortex and hippocampus of mice subjected to TBI. Mice (n=6/group) were subjected to sham surgery or closed-cortical impact surgery, and were treated 30 minutes after surgery with either DMSO vehicle (Veh) or acetazolamide (15 mg/kg, AZA). At 1-day after injury, mice were sacrificed. Representative images of Nissl-stained sections are shown of mice subjected to (a) sham surgery and vehicle treatment, (b) TBI with vehicle treatment, (c) sham surgery with acetazolamide, or (d) TBI with acetazolamide treatment. Inset boxes detail higher magnification views of the indicated regions in either the CA1 region of the hippocampus or the cortex. Representative cells have been circled in red. Cell body sizes were measured and were plotted either as (e) a dot plot, with the mean indicated in red or as (f) a histogram plot. Significance was measured using ANOVA followed by Tukeyâ€™s HSD test.
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