Cytochrome c: A Multifunctional Protein Combining Conformational Rigidity with Flexibility
Figure 22
Charge transfer band region of the absorption spectrum of 0.5 mM ferricytochrome c (equine) measured between 13,500 and 17,000 cm−1 in 0.1 mM potassium phosphate buffer. Prior to the measurement the oxidized protein was exposed to alkaline conditions at pH 11.5 for one week. The label CT1 exhibits the region of the so-called 695 nm charge transfer band, which is practically absent in the displayed spectra. CT2 is assignable to a heme iron transition in high and quantum mixed heme irons. The spectrum was taken from [147] with permission.
