5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice

Figure 4

The effect of aging on 5-LOX DNA methylation in the region promoter-5^′UTR. The methylation-sensitive endonuclease assay with the four endonucleases—AciI, BstUI, HinP1I, and HpaII—was performed as described in the text and indicated in Figure 1. The heart and brain (C: cerebellum, FC: frontal cortex, H: hippocampus) tissue samples were obtained from 2-month-old mice (open bars) and 22-month-old mice (closed bars). The results are expressed as units (the coefficient of variation values multiplied by a factor of 1000, see text for details) and shown as mean ± S.E.M (heart, C, and H: 𝑛=5; FC: 𝑛=10). *𝑃<.05, ^**𝑃<.01, and ^***𝑃<.001 versus the corresponding values in samples from 2-month-old mice (𝑡-test).

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