5-Lipoxygenase DNA Methylation and mRNA Content in the Brain and Heart of Young and Old Mice

Table 1

Primer sequences used to assay 5-LOX, DNMT1, DNMT3a, and cyclophilin (reference) mRNA.

mRNA	Gene bank accessio number	Primers	Fragment size (bp)
5-LOX	NM_009662	F: 5^′-ATTGCCATCCAGCTCAACCAAACC-3^′	178
		R: 5^′-TGGCGATACCAAACACCTCAGACA-3^′
DNMT1	NM_010066	F: 5^′-AGTGCAAGGCGTGCAAAGATATGG-3^′	150
		R: 5^′-TGGGTGATGGCATCTCTGACACAT-3^′
DNMT3a	NM_007872	F: 5^′-ACAAGAATGCTACCAAAGCAGCCG -3^′	200
	NM_153743	R: 5^′-TGAGAACTTGCCATCTCCGAACCA-3^′
Cyclophilin	X52803	F: 5^′- AGCATACAGGTCCTGGCATCTTGT –3^′	153
		R: 5^′- AAACGCTCCATGGCTTCCACAATG -3^′