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Neural Plasticity
Volume 2009, Article ID 852492, 7 pages
Research Article

Directed Fiber Outgrowth from Transplanted Embryonic Cortex-Derived Neurospheres in the Adult Mouse Brain

1HNO Klinik, ZLF 411, University of Basel, Hebelstr. 20, 4031 Basel, Switzerland
2Department of Biomedicine, Anatomical Institute, University of Basel, Pestalozzistr. 20, 4056 Basel, Switzerland

Received 17 July 2009; Revised 22 October 2009; Accepted 19 November 2009

Academic Editor: Mayank Dutia

Copyright © 2009 Vesna Radojevic and Josef P. Kapfhammer. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Neural transplantation has emerged as an attractive strategy for the replacement of neurons that have been lost in the central nervous system. Multipotent neural progenitor cells are potentially useful as donor cells to repopulate the degenerated regions. One important aspect of a transplantation strategy is whether transplanted cells are capable of fiber outgrowth with the aim of rebuilding axonal connections within the host brain. To address this issue, we expanded neuronal progenitor from the cortex of embryonic day 15 ubiquitously green fluorescent protein-expressing transgenic mice as neurospheres in vitro and grafted them into the entorhinal cortex of 8-week-old mice immediately after a perforant pathway lesion. After transplantation into a host brain with a lesion of the entorhino-hippocampal projection, the neurosphere-derived cells extended long fiber projections directed towards the dentate gyrus. Our results indicate that transplantation of neurosphere-derived cells might be a promising strategy to replace lost or damaged axonal projections.