Figure 4: Morphological analysis of dendritic spines. (a) Black lines indicate measurements of spine length. White lines indicate trajectories across which profiles of fluorescence were obtained from the spine head and neck. Scale bar: 2 μm. (b) Measures of spine head diameter, neck length, and relative neck diameter did not differ significantly between WT and prestin KO animals in layer 2/3 ( 𝑃 > . 0 5 ). (c) Spines can be loosely classified into morphological “types” by comparing their relative head, neck, and length dimensions. The complete overlap of WT spines with KO spines indicates that there is no difference in spine type distribution between genotypes.