Neural Plasticity / 2011 / Article / Fig 5

Research Article

Diacylglycerol Signaling Underlies Astrocytic ATP Release

Figure 5

OAG-induced ATP release from astrocytes appears to be vesicular. (a) Incubation of cultured astrocytes with 100 nM Bafilomycin A1, an inhibitor of the vesicular H+  ATPases that set up the proton gradients necessary for vesicle filling, greatly reduced OAG-induced astrocytic ATP release (bafilomycin 𝑛 = 8 , control 𝑛 = 5 ; 𝑑 1 𝑃 = 0 . 0 1 , 𝑑 2 𝑃 = 0 . 0 2 , 𝑑 3 𝑛 = 0 . 0 0 4 ). This effect was partially washed out after 15 minutes ( 𝑑 2 bafilomycin versus 𝑑 3 wash 𝑃 = 0 . 0 2 4 ). (b) Bafilomycin treatment did not affect the function of astrocytic ATP receptors, as the calcium response to 1 μM ATP was unaffected by bafilomycin treatment (bafilomycin 𝑛 = 5 , control 𝑛 = 5 , 𝑃 = 0 . 9 ). (c) The gap junction and hemichannel blocker carbenoxolone (CBX), at 50 μM, resulted in an increase in astrocytic ATP release (control 𝑛 = 1 4 , CBX 𝑛 = 5 ; 𝑑 2 𝑃 = 0 . 0 3 , 𝑑 3 𝑃 = 0 . 0 2 ).
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537659.fig.005b
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