Identification and Characterization of Two Novel RNA Editing Sites in grin1b Transcripts of Embryonic Danio rerio
Figure 5
Genomic products; E5, E6. PCR amplification of regions within the grin1b gene corresponding to E5 and E6 potential RNA editing sites. As predicted by the DS Gene computer program (Accelrys), primers designed to target the genomic E5 sequence (ZF.Grin1bE5.F1 and ZF.Grin1bE5.R1) produced a strong band at around 200 nucleotides. Primers aimed at amplifying the genomic E6 sequence (ZF.Grin1bE6.F1 and ZF.Grin1bE6.R1) produced a strong band at around 220 nucleotides. Both of these bands were extracted for sequencing reactions. Amplicons from genomic DNA show no evidence of A/G polymorphism (A only). Dashed black lines represent noise levels per sample.
