ZD7288 Enhances Long-Term Depression at Early Postnatal Medial Perforant Path-Granule Cell Synapses
Figure 4
L-NAME enhances LFS-induced LTD in early postnatal, but not adult, rats. (a) The time course of the fEPSP amplitude following LFS in early postnatal rats (P9–15) shows robust LTD (closed symbols) as compared to time-control experiments without LFS (open symbols). L-NAME (100 μM) was applied 10 min prior to LFS (indicated by a lack bar). Representative fEPSPs were taken at the beginning (indicated by “1”) and at the end (indicated by “2”) of the experiment. (b) The time course of the fEPSP amplitude following LFS in adult rats (P30–60) also shows LTD (closed symbols) as compared to time-control experiments without LFS (open symbols). L-NAME (100 μM) was applied 10 min prior to LFS (indicated by a lack bar). Note that less LTD is obtained compared to early postnatal animals (panel A). Representative fEPSPs were taken at the beginning (indicated by “1”) and at the end (indicated by “2”) of the experiment. (c) The bar graph summarizes the LTD levels for slices treated with L-NAME (closed bars). For the sake of clarity, data from Figures 1(e) and 2(d) is also shown in order to illustrate non-L-NAME conditions (open bars). The number of slices is given in parentheses (number of LFS-treated slices/number of non-LFS-treated slices). (d) The bar graph summarizes the paired-pulse ratio of all experimental groups recorded at the beginning of each experiment, that is, before L-NAME and/or LFS were applied to the slices. Note that the PPR was similar in all groups. All data are mean ± SEM.
