Regulation of Ca<sup>2+</sup>/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal

<table>Distribution histograms of postsynaptic profiles containing different numbers of CaMKII (a) and pCaMKII (b) immunogold particles within the PSD. Histograms represent the average relative frequencies of synapses lacking (0 particles) CaMKII or pCaMKII immunogold particles or containing 1 to 5 immunogold particles. There was a significant decrease in the fraction of FZP-withdrawn synapses containing two CaMKII immunogold particles (CON: <svg height="11.2875" id="M65" style="vertical-align:-0.3003pt" version="1.1" viewbox="0 0 90.6875 11.2875" width="90.6875" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,8.222,10.862)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,12.098,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,20.257,10.862)"><use xlink:href="#x39"></use></g><g transform="matrix(.017,-0,0,-.017,28.416,10.862)"><use xlink:href="#x34"></use></g><g transform="matrix(.017,-0,0,-.017,40.35,10.862)"><use xlink:href="#xB1"></use></g><g transform="matrix(.017,-0,0,-.017,54.102,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,62.261,10.862)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,66.137,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,74.296,10.862)"><use xlink:href="#x33"></use></g><g transform="matrix(.017,-0,0,-.017,82.455,10.862)"><use xlink:href="#x30"></use></g>
</svg>, <svg height="10.925" id="M66" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 36.237499 10.925" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.65)"><use xlink:href="#x1D45B"></use></g><g transform="matrix(.017,-0,0,-.017,13.305,10.65)"><use xlink:href="#x3D"></use></g><g transform="matrix(.017,-0,0,-.017,28.008,10.65)"><use xlink:href="#x35"></use></g>
</svg>; FZP: <svg height="11.2875" id="M67" style="vertical-align:-0.3003pt" version="1.1" viewbox="0 0 90.6875 11.2875" width="90.6875" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,8.222,10.862)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,12.098,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,20.257,10.862)"><use xlink:href="#x31"></use></g><g transform="matrix(.017,-0,0,-.017,28.416,10.862)"><use xlink:href="#x32"></use></g><g transform="matrix(.017,-0,0,-.017,40.35,10.862)"><use xlink:href="#xB1"></use></g><g transform="matrix(.017,-0,0,-.017,54.102,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,62.261,10.862)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,66.137,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,74.296,10.862)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,82.455,10.862)"><use xlink:href="#x35"></use></g>
</svg>, <svg height="10.925" id="M68" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 36.237499 10.925" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.65)"><use xlink:href="#x1D45B"></use></g><g transform="matrix(.017,-0,0,-.017,13.305,10.65)"><use xlink:href="#x3D"></use></g><g transform="matrix(.017,-0,0,-.017,28.008,10.65)"><use xlink:href="#x35"></use></g>
</svg>; <svg height="11.375" id="M69" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,15.582,11.112)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,30.286,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,38.446,11.112)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,42.321,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,50.481,11.112)"><use xlink:href="#x35"></use></g>
</svg>, Mann-Whitney <i>U</i>-test), whereas no significant difference was detected in the distribution of pCaMKII immunogold particles.</table>

Neural Plasticity

fig3

Figure 3

Figure 3: Regulation of Ca<sup>2+</sup>/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal 

Figure 3 | Regulation of Ca2+/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal 