Activity-Dependent Callosal Axon Projections in Neonatal Mouse Cerebral Cortex
Figure 1
Visualization of callosal axon projections and an effect of activity reduction. (a) An in utero electroporation-mediated gene transfer method [38] was used to unilaterally express EGFP in layer 2/3 cortical neurons (electroporated side). EGFP-labeled callosal axons extend through the corpus callosum, and project densely to a narrowly restricted region in the contralateral cortex (arrow). Scale bar, 500 μm. (b) EGFP-labeled control callosal axons show lamina specific projection pattern. (c) Reduction of neuronal activity in callosal projection neurons disturbs their axonal projections. Adapted from [38]. Scale bar, 200 μm.
