Blockade of Lysosomal Acid Ceramidase Induces GluN2B-Dependent Tau Phosphorylation in Rat Hippocampal Slices
Figure 2
D-NMAPPD treatment is associated with increased phosphorylation of GluN2B receptor subunits. Levels of total or phosphorylated NMDA receptor subunits were estimated by Western blotting on cell extracts obtained from hippocampal slices treated with d-NMAPPD (25 μM) for 3 h. (a) Phosphorylated GluN2B levels at Tyr1472 and Tyr1336 epitopes were expressed relative to total GluN2B subunit levels. (b) Phosphorylated GluN1 levels at Ser896 and Ser897 epitopes were expressed relative to total GluN1 subunit levels. Summary data are shown in the bar graph (means ± SEM of 6 different experiments). Since these experiments were performed independently, statistical significance was estimated by conventional unpaired -test. , , and d-NMAPPD (3 h) versus the controls. Note the ability of d-NMAPPD to strongly enhance GluN2B receptor phosphorylation at residue Tyr1472.
