Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg²⁺-Induced Epileptic Hippocampal Slice Models

Figure 1

Slices mounted on MEA and low-Mg²⁺-induced epileptiform discharges. (a) An image of an example of a hippocampal slice mounted on MEA. The black dots indicate the electrodes (60 channels) of the MEA, with each electrode number labeled at its top left corner. (b) Low-Mg²⁺-induced epileptiform discharges recorded by the MEA. Each data window represented the recording from one electrode, with each electrode number labeled at its top right corner. (c) A portion of raw data recorded by one electrode (number 87), which showed epileptiform discharges in the CA3b region, with epileptiform activities presented using different time scales. (d) An image of an example of combined EC-hippocampal slice mounted on MEA. (e) Low-Mg²⁺-induced ictal discharges recorded by the MEA. (f) A portion of raw data recorded by one electrode (number 67), which showed epileptiform discharges in the CA3b region of the combined slice. There were two types of epileptiform discharges recorded in the hippocampal area: interictal and ictal discharges. Each discharge cycle consisted of several minutes of interictal discharges and tens of seconds of ictal discharge. After a resting period, this alternating interictal and ictal discharge pattern repeated.