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Neural Plasticity
Volume 2014, Article ID 584314, 12 pages
Research Article

Abeta(1-42) Enhances Neuronal Excitability in the CA1 via NR2B Subunit-Containing NMDA Receptors

1Department of Medical Chemistry, University of Szeged, Szeged 6726, Hungary
2Biological Research Center—Biochemistry, Hungarian Academy of Sciences, Temesvari Körút 32, Szeged 6726, Hungary

Received 13 June 2014; Revised 5 August 2014; Accepted 17 August 2014; Published 3 September 2014

Academic Editor: Lucas Pozzo-Miller

Copyright © 2014 Edina Varga et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. CNQX abolished evoked fEPSPs even under condition that promotes NMDAR activation (in low Mg2+ ACSF)

Supplementary Figure 2. Low concentration of AMPA (0.05 μM) increased basal activity which hindered the effective detection of spikes. Figure shows representative traces before (left) and after (right) AMPA application.

Supplementary Figure 3. Small concentration of NMDA (0.5 μM), which was reported to activate extrasynaptic NMDARs, did not affect fEPSPs

Supplementary Figure 4. Abeta(1-42) samples used for the recordings contained clearly detectable SDS-stable trimers (≈15 kDa) and higher molecular weight protofibrils. Staining with OC antibody, which is specific for species having beta-sheet conformation, shows that the small low-n oligomers are of prefibrillar nature.

  1. Supplementary Material