Research Article

Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats

Figure 3

Delayed putrescine supplementation significantly increases SC implant size. To determine the effect of putrescine on the size of the implant, immunostaining for p7 was performed and the area of immunoreactivity quantified. Images from representative animals show the extent of p7 immunoreactivity among the different SC implant groups: (a) vehicle control, (b) immediate putrescine, and (c) delayed putrescine. Scale bar = 500 μm. Dotted white lines indicate the extent of the SC implant. Stereological quantification of the implant area shows an increase in size when putrescine is administered in a delayed fashion, but not acutely, as compared to the SC with vehicle control (d). Statistical significance indicated at compared to SCs with vehicle control group.
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