In Vitro Studies of Neuronal Networks and Synaptic Plasticity in Invertebrates and in Mammals Using Multielectrode Arrays

Table 1

Summary of the plasticity protocols used in mammalian neuronal cultures.

Reference	Year	Plasticity protocol
Maeda et al. [139]	1998	20 bursts at 0.2 Hz, each with 11 pulses at an intraburst frequency of 20 Hz delivered from 5 electrodes
Jimbo et al. [140]	1998	11 bursts at 0.2 Hz, each with 11 pulses at an intraburst frequency of 20 Hz
Jimbo et al. [141]	1999	10 bursts at 0.2 Hz, each with 11 pulses at an intraburst frequency of 20 Hz
Tateno and Jimbo [142]	1999	10 bursts at 0.2 Hz, each with 11 pulses at an intraburst frequency of 20 Hz, also delivered from a pair of electrodes
Shahaf and Marom [143]	2001	Adaptive stimulation between a pair of electrodes at 1–3 s intervals repeated until the desired response is achieved (or for 10 min max)
Ruaro et al. [144]	2005	Bursts of 100 pulses delivered at the frequency of 250 Hz from 15 electrodes recreating an “L-shape”
Wagenaar et al. [25, 145]	2006	150 trains of 20 pulse pairs. Bursts suppressed by means of a high frequency (50 Hz) distributed stimulation (not during tetanus delivery)
Chiappalone et al. [20]	2008	Jimbo protocol with additional trains of pulses at 0.2 Hz falling in the middle of the tetanic burst
Le Feber et al. [146]	2010	Slow electrical stimulation (0.2–0.33 Hz)