Neural Stem Cell Transplant-Induced Effect on Neurogenesis and Cognition in Alzheimer Tg2576 Mice Is Inhibited by Concomitant Treatment with Amyloid-Lowering or Cholinergic 7 Nicotinic Receptor Drugs

Figure 2

hNSC transplantation increases the number and the maturation of new neurons in the dentate gyrus. (a) Doublecortin- (DCX-) labeled cells in the dentate gyrus (DG) of Tg2576 mice were characterized according to their numbers and the level of maturation and dendritic arborization. (b) The total number of DCX+ cells, (c) the number of DCX+ cells possessing dendrites, and (d) the average number of dendritic branches on DCX+ cells in the DG in mice receiving SHAM (vehicle only) transplantation and saline (SHAM + SAL) or mice receiving hNSC transplantation and saline (hNSC + SAL), JN403 (hNSC + JN), or (+)-phenserine (hNSC + PHEN). (e) Correlation between the number of DCX+ cells with dendrites and the difference in time taken to find the position of the platform ( latency; follow-up probe test values minus baseline acquisition values) in the Morris water maze task in groups of Tg2576 mice treated with hNSC + SAL, hNSC + JN, and hNSC + PHEN (; , ). Representative images of immunostaining with DCX+ cells in the DG of TG2576 mice at (f) 10x and (g) 20x magnification. compared to SHAM + SAL, and compared to hNSC + SAL. The data are expressed as means ± SEM.

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