Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis

Figure 2

Basic electrophysiological characteristics and evoked excitatory postsynaptic currents (EPSCs) of transfected neurons. (a) Dual-cell patch-clamp recorded hippocampal neurons transfected with siRNA or a scrambled sequence (negative control, NC) (left, green) and nontransfected neurons (right, gray). (b) Representative traces from neurons. Transfected and nontransfected neurons exhibit many action potentials (APs) following 400 ms, 200 pA current injection (left panel). EPSC evoked in a nontransfected neuron by a single AP from a transfected neuron (right panel). (c) Input-output relationship of APs evoked by 400 ms current injection at various intensities in normal neurons (blank, ), NC (), and siRNA-transfected neurons (, including five Endo1 siRNAs, five Endo2 siRNAs, and six Endo3 siRNAs). (d) EPSC amplitude evoked in a nontransfected neuron by a single AP in a neuron transfected with NC (), Endo1 siRNA (), Endo2 siRNA (), and Endo3 siRNA ().

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