PKA Inhibitor H89 (N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulfonamide) Attenuates Synaptic Dysfunction and Neuronal Cell Death following Ischemic Injury

Figure 7

The measurement of BDNF and Bcl2 mRNA level in Neuro2A cells after H/R-induced injury. (a) BDNF and (b) Bcl2 mRNA levels were measured by using RT-PCR. The H89 pretreatment group showed higher mRNA levels of (a) BDNF and (b) Bcl2 compared to the hypoxia reperfusion injury group. Data were expressed as mean ± S.E.M, and each experiment included 3 repeats per condition. GAPDH was used as a control. Differences were considered significant at and . Normal: the normal control group, H/R: 4 hr hypoxia and 18 hr reperfusion injury group, H89: 2 hr PKA inhibitor H89 treatment group before 4 hr hypoxia and 18 hr reperfusion injury.