Prophylactic Subacute Administration of Zinc Increases CCL2, CCR2, FGF2, and IGF-1 Expression and Prevents the Long-Term Memory Loss in a Rat Model of Cerebral Hypoxia-Ischemia

Figure 1

Effect of subacute administration of zinc on CCL2 expression in cerebral cortex-hippocampus of the rat. RT-PCR was used to determine mRNA levels of CCL2 (323 bp) and GA3PDH (420 bp). ((a) and (b)) Showing representative photographs of ethidium-bromide-stained RT-PCR products fractionated on 2% agarose gel and the respective densitometry analysis. ((c) and (d)) Showing the CCL2 protein levels using ELISA. Each value represents the mean ± SEM of 5 independent experiments made in triplicate. (1) Zn96h, rats injected with ZnCl₂ (one dose every 24 h during 4 days). (2) Zn96h + CCAO, rats treated with zinc before 10 min of common carotid artery occlusion (CCAO). (3) CCAO, rats with CCAO only. (4) Sham group, rats with mock CCAO. (5) Untreated rats. , significant when compared with the control group; ANOVA test and post hoc Dunnett’s test. , significant when compared between groups; unpaired Student’s -test. .