Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B

Figure 1

Rotational behaviour and tyrosine-hydroxylase immunocontent in rats administered an intrastriatal 6-OHDA injection. (a) the number of ipsilateral rotations after a methylphenidate challenge. Tyrosine-hydroxylase immunocontent in the striatum (b) and substantia nigra (c) at 1st, 7th, or 21st days after 6-OHDA intrastriatal administration. The striatum and substantia nigra were dissected and homogenized for the measurement of the tyrosine-hydroxylase by Western Blotting analysis. Each value represents the mean ± standard error from at least 4 rats per group. ^∗The 6-OHDA-group is significantly different than the sham/vehicle-group (two-way ANOVA, ), in (a), (b), and (c). from 6-OHDA-lesioned rats at days 1 and 7; from 6-OHDA-group at 1 day; from 6-OHDA-group 1 day after and different, from sham-group 21 days after surgery (two-way ANOVA followed by Tukey’s post hoc test, ). Panel (d) shows a representative TH-immunohistochemistry for dopamine-producing cells in the ipsilateral and contralateral sides of the substantia nigra (SN) pars compacta in a 6-OHDA-lesioned rat 21 days after surgery. Scale bar = 10 μm.

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