Multiscale Modeling Indicates That Temperature Dependent [Ca²⁺]_i Spiking in Astrocytes Is Quantitatively Consistent with Modulated SERCA Activity

Table 1

Physiologic parameters of the in silico cell used in simulations. Note that the GCA uses a linearized reaction diffusion system leading to rates for and .

Physiological cell parameter values for GCA simulations
	10 μm	Cell radius
	5	Volume ratio between cytosol and ER
	220 μm^2/s	Diffusion coefficient of cytosolic
	70 μm^2/s	Diffusion coefficient of lumenal
	95 μm^2/s	Diffusion coefficient of mobile buffer
	55 nM	Cytosolic base level
	800 μM	Endoplasmic reticulum base level
	80 μM	concentration
	52 μM	Total mobile buffer concentration (EGTA)
	0.3 (μMs)^−1	On rate of the mobile buffer
	1.5 s^−1	Dissociation rate of the mobile buffer
	30 μM	Total immobile buffer concentration
	600 (μMs)^−1	On rate of the immobile buffer
	s^−1	Dissociation rate of the immobile buffer
	≈0.01 s^−1	Leak flux constant given by varied , and