Fractalkine Signaling and Microglia Functions in the Developing Brain

Figure 4

Impaired microglia motility in developing CX3CR1 deficient mice. (a) Two-photon images in an acute slice of a P7 Cx3cr1^+/eGFP mouse showing the dynamics of microglial processes and soma after the insertion (at time 0 min, not shown) of a pipette (red dot) containing 2-MeSADP (100 µM). Yellow arrowheads indicate the soma of 2 microglial cells moving toward the pipette. Green arrow indicates a retracting process. Calibration bar is 10 µm. (b) Comparison of the mean velocity of microglia soma toward the 2-MeSADP-containing pipette for Cx3cr1^+/eGFP (50 cells, 6 experiments) Cx3cr1^+/eGFP (42 cells, 5 experiments) animals (, , Mann-Whitney test).

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