Glial PlasticityView this Special Issue
Review Article | Open Access
Zila Martínez-Lozada, Arturo Ortega, "Glutamatergic Transmission: A Matter of Three", Neural Plasticity, vol. 2015, Article ID 787396, 11 pages, 2015. https://doi.org/10.1155/2015/787396
Glutamatergic Transmission: A Matter of Three
Glutamatergic transmission in the vertebrate brain requires the involvement of glia cells, in a continuous molecular dialogue. Glial glutamate receptors and transporters are key molecules that sense synaptic activity and by these means modify their physiology in the short and long term. Posttranslational modifications that regulate protein-protein interactions and modulate transmitter removal are triggered in glial cells by neuronal released glutamate. Moreover, glutamate signaling cascades in these cells are linked to transcriptional and translational control and are critically involved in the control of the so-called glutamate/glutamine shuttle and by these means in glutamatergic neurotransmission. In this contribution, we summarize our current understanding of the biochemical consequences of glutamate synaptic activity in their surrounding partners and dissect the molecular mechanisms that allow neurons to take control of glia physiology to ensure proper glutamate-mediated neuronal communication.
Glutamate (Glu) the main excitatory neurotransmitter in the nervous system requires the involvement of neurons and glia cells to elicit its function as a neurotransmitter in an example of what is nowadays known as a tripartite synapse. Although detailed analysis of neuronal consequences of Glu exposure is regularly reviewed [1–3], the cellular and molecular impact of Glu in glia cells and its outcome in terms of synaptic communication are much less considered. Traditionally, it has been thought that Glu ejects its functions through the activation of specific membrane receptors classified in two main groups: ionotropic (iGluRs) and metabotropic (mGluRs). However, recent findings suggest the participation of Glu transporters in the signaling transactions triggered by this amino acid. Needless to say, both receptors and transporters are expressed in glia cells.
In the following sections, an insight into the signaling strategies used by this amino acid that end up into an efficient neuronal communication by means of altering the glial proteome is summarized and discussed.
2. Glutamate Receptors
Based on the sequence and transduction similarities, two main subtypes of Glu receptors have been defined: iGluRs and mGluRs. iGluRs are ligated-coupled ion channels that were originally classified according to their pharmacological profile into 5-methyl-4-isoxazole propionate (AMPA), kainate (KA), and N-methyl-D-aspartate (NMDA) receptors . In terms of their molecular structure, each of these subtypes is composed of four subunits encoded by different genes. AMPA receptors are composed of different combinations of GRIA1 (GluA1), GRIA2 (GluA2), GRIA3 (GluA3), and GRIA4 (GluA4). Each combination displays different cation channel properties; for instance, the sole presence of one GluA2 subunit favours Na+ permeability. AMPA receptors lacking the GluA2 subunit are Ca2+-permeable , like those expressed in radial glia cells [6, 7]. KA receptors are composed of four subunits out of GluK1-5. While AMPA and KA receptors are homo- or heterooligomers NMDA receptors are formed as heteromers since in order to be functional they must contain at least one GluN1 subunit, with the other subunits of the channel being GluN2A-D or GluN3A-B subunits. The molecular diversity of these receptors is enormous, since most of these ionotropic subunits undergo RNA edition as well as splicing .
In contrast, mGluRs are members of class C of G-protein coupled receptors (GPCR) and had been classified based on sequence homology, G-protein coupled and pharmacology in three groups. Group I is comprised of mGluR 1 and mGluR 5 and is coupled to stimulation of phospholipase C with the consequent release of intracellular Ca2+, group II contains mGluR 2 and mGluR 3 and is coupled to adenylate cyclase inhibition, and group III consists of mGluR 4, mGluR 6, mGluR 7, and mGluR 8; as group II, group III is also linked to adenylate cyclase inhibition. These three groups are activated by specific agonist: for group I (RS)-3,5-dihydroxyphenylglycine (DHPG) and for group II (S)-4-carboxy-3-hydroxy-phenylglycine (S)-4C3HPG, while for group III L-(+)-2-amino-4-phosphonobutyric acid (L-AP4) .
3. Glial Glu Receptors
Glial cells express different types of Glu receptors depending on the brain region and the differentiation stage . For example, AMPA receptors are expressed in astrocytes throughout the entire brain; however, their properties differ due to the differential GluA subunits expression. In cerebellum, retina, and brainstem, glial AMPA receptors lack GluA2 subunit; therefore, these channels are Ca2+-permeable (Table 1) [11–14]. AMPA-mediated Ca2+ influx has an important role in glial metabolism and structure. It has been demonstrated that AMPA receptors regulate the protein repertoire in Bergmann glial cells at transcriptional and translational levels, as will be discussed more broadly ahead [15–17]. Moreover, when Bergmann glia AMPA receptors are rendered Na+-permeable, their fusiform morphology changes through the retraction of the glial processes .
In contrast, although the transcripts and protein of various KA receptors subunits have been described in glial cells, no functional evidence of these receptors has been reported . It has been speculated that KA receptors also participate in glial response to Glu since an increase of GluKs subunits expression is present in reactive astrocytes (Table 1) .
The expression of the seven known NMDA subunits has been demonstrated in glial cells (Table 1) [21–23], and glial NMDA receptors have peculiarities compared with neuronal NMDA receptors; glial NMDA receptors present a very weak Mg2+ blockage and a lower Ca2+ permeability [19, 24, 25].
In glial cells, mGluRs are also present; in fact astrocytes express all of the described subtypes, and mGluRs 1 and 5 from group I are linked to the activation of phospholipase C, while mGluRs 2 and 3 from group II and mGluRs 4, 6, 7, and 8 from group III are coupled to the inhibition of adenylate cyclase (Table 1) . Calcium waves derived from stimulation of mGluR group I in glial cells have been shown and are considered as an important mechanism for Glu-dependent intracellular Ca2+ regulation in glial cells .
4. Membrane Glu Transporters
Glu extracellular levels are tightly regulated by a family of Na+-dependent Glu transporters known as excitatory amino acid transporters (EAATs) . Five subtypes of Glu transporters have been characterized thus far and have been named EAATs 1–5. While EAAT 1 and EAAT 2 are regarded as glia specific, EAATs 3, 4, and 5 are present in neurons. The glial transporters EAAT 1, also known as Na+-dependent Glu/aspartate transporter (GLAST), and EAAT 2 (Glu transporter 1 (GLT-1)) are responsible of approximately 80–90% of Glu uptake activity in the brain , reflecting not only that glia cells outlast neurons in a 1 : 10 proportion, but also that these proteins are profusely expressed in glia cells. The neuronal transporters EAATs 3–5 have a more restricted distribution, EAAT 3 is expressed mainly in hippocampal neurons, and EAAT 4 is present in Purkinje cells in the cerebellum while EAAT 5 has been found in retina . It should be noted, however, that EAAT 2 expression in neurons and EAAT 4 presence in astrocytes have also been documented [78, 80].
Glial Glu transporters are abundant; in fact it has been calculated that GLT-1/EAAT 2 represents 2% of total brain protein. While GLAST/EAAT 1 is preferentially expressed in cerebellum, retina, and olfactory bulb, GLT-1 is abundant in all other brain areas. During development, GLAST is the most abundant glial Glu transporter, and as such it has been widely used as a glial marker in numerous ontogeny studies (Table 2) .
These transporters have been traditionally implicated in Glu turnover through the so-called Glu/glutamine (Gln) shuttle. Once this amino acid is removed from the synaptic space, it is rapidly converted to Gln through the action of Gln synthetase [81, 82]. Sodium-dependent neutral amino acid transporters (SNATs) mediate both the glial release and the neuronal uptake of Gln, which once in the neuronal compartment is deaminated to regenerate Glu that is charged into the synaptic vesicles due to the action of the vesicular transporters (VGLUT).
A biochemical and physical coupling of GLAST with SNAT 3 was found in Bergmann glial cells, and we could demonstrate that the Na+ influx through GLAST activity is coupled to the Gln release mediated by SNAT 3 ; these results suggest that glial cells surrounding glutamatergic synapses sense neuron-derived Glu to promote a more efficient Glu recycling and in consequence an enhanced neuronal communication.
Recent evidences suggest that Glu transporters might also participate in the signaling transactions triggered by this amino acid. More than two decades ago, Amara and coworkers demonstrated a Glu-dependent Ca2+ influx via an unconventional mechanism that involved Glu transporters rather than Glu receptors in pituitary GH3 cells ; later on, different groups reported that Glu transporters translocation to the plasma membrane is regulated by the transporter itself [85, 86]. These findings added a novel regulatory mechanism for EAATs, recently expanded to include the membrane diffusion of the transporters which has been shown to modify the kinetics of excitatory postsynaptic currents .
In this context, Glu transporters have receptor-like properties; for example, in rat cortical astrocytes, L-Glu, D- and L-Aspartate, and transportable Glu uptake inhibitors increase p42/ phosphorylation . Glu transporters activity also impacts the PI3K/Akt/mTOR pathway, an important mechanism to regulate protein synthesis after glutamatergic stimulation [89, 90]. It also has been reported that Glu transporters have physical interaction with Na+/K+-ATPase and operate as a functional macromolecular complex to regulate glutamatergic neurotransmission [91–93].
5. Vesicular Glu Transporters
While the Glu release by glial cells has been well documented, the mechanisms involved in this release are still controversial. One of the proposed mechanisms is the activation of the reversal mode of EAATs ; the other one is the Glu vesicle-mediated, Ca2+-dependent release .
Three isoforms of vesicular Glu transporters (VGLUTs 1, 2, and 3) have been cloned and characterized in the brain; these isoforms have differential distribution and distinct roles, as expected [96, 97]. The expression of VGLUTs in neurons is well documented [97, 98]; however their presence in glial cells is still under discussion.
The first report of the presence of functional VGLUTs in glial cells was shown in 2004 (Table 2) . Using rat visual cortex, cultured astrocytes these authors suggest the expression of VGLUTs based on the fact that pharmacological inhibition of VGLUTs reduces a Ca2+-dependent exocytosis Glu release from astrocytes. Moreover, VGLUT 3 overexpression results in an enhanced Ca2+-dependent Glu release . It is important to mention that the biochemical machinery (for example, synaptobrevin II), needed for a vesicular Glu release has also been detected in cultured astrocytes . A stimulus and Ca2+-dependent Glu release has been reported  favouring the idea of gliotransmitters regulated release. There are different subtypes of vesicles storing amino acids, peptides, and ATP in astrocytes. Despite these findings, a biochemical evidence of the VGLUTs expression in glial cells is still absent; Li and collaborators evaluated VGLUTs expression using Western blots and single-vesicle imaging by total internal reflection fluorescence microscopy concluding that their findings could not support an irrefutable evidence of VGLUTs expression in glial cells . In this scenario, the molecular mechanisms involved in Ca2+-dependent Glu release are uncertain.
6. Glu-Dependent Gene Expression Regulation in Glia Cells
6.1. Transcriptional Control
The ability of glial cells to modify their transcriptional profile in response to Glu was one of the first questions asked after the expression of functional Glu receptors was fully characterized . The increase in intracellular Ca2+ associated with Glu exposure in a plethora of glia cells preparations led to the search of the expression and DNA binding of several transcription factors such as Fos, Jun, and the cAMP response element-binding protein (CREB) [104–106]. The identification and characterization of downstream genes regulated by Glu in glia cells have started to emerge and systematic transcriptional studies have also been undertaken, for example, in Bergmann glia . The overall picture is that the transcriptional pattern upon Glu stimulation varies from different glia subtypes, and in that sense the signaling cascade that regulates such effect is specific [108, 109].
6.2. Translational Control
Translation represents the final step in gene expression regulation. Translational control offers the advantage of rapid response to external stimulus to change gene expression profiles without the requirement of mRNA synthesis and transport. Protein synthesis is the most energy demanding process in cell physiology and given the fact that glia cells that surround glutamatergic synapses are engaged in Glu removal, a biochemical phenomenon that relies on the activity of the Na+/K+-ATPase, the idea that Glu could regulate protein synthesis has long been attractive. Indeed, Glu induces a biphasic effect in overall protein synthesis in cultured Bergmann glia . Glu treatment modifies [35S]-methionine incorporation into newly synthesized polypeptides in a time dependent event marked by a decrease in [35S]-methionine incorporation 15 min after Glu exposure, but after 30 min this phenomenon starts to revert, returning to basal levels after 120 min. The ribosomal transit time (RTT), meaning the average time that a cell takes to synthetize a polypeptide , is augmented 7-fold in Glu-treated cultured Bergmann glia, event that is reverted after 120 min .
Translational control is mainly mediated by phosphorylation of several components of the translational machinery . The initiation phase is a recurrent target of regulation, through the posttranslational modification of eukaryotic initiation factors (eIFs). The initiator methionyl-tRNA is conveyed to the ribosome assembly by eukaryotic initiation factor 2 (eIF2) complexed with GTP. The conversion of inactive eIF2-GDP to active eIF2-GTP by eIF2B is regulated by phosphorylation. eIF2 has three subunits (α, β, and γ). Glu exposure leads to serine 51 eIF2α phosphorylation, modification that converts eIF2 from a substrate to a competitive inhibitor of eIF2B . This phosphorylation does not inhibit the general function of eIF2 but renders the protein defective in recycling, resulting in the inhibition of the initiation phase of protein synthesis.
Glu exposure also regulates translation elongation, again, decreasing protein synthesis by the inhibition of the ribosomal translocation. It should be noted that regulation of elongation process is indicative of a transient effect since the mRNA remains attached to the ribosomes allowing an immediate reinitiation of the translation process, as shown in Bergmann glia .
In summary, a cascade of phosphorylation/dephosphorylation of translation factors is involved in the Glu biphasic translational control in cultured Bergmann glia. Exposure to this excitatory amino acid reduces in the first 15 min [35S]-methionine incorporation into trichloroacetic acid- (TCA-) precipitable polypeptides. Thereafter, a gradual recovery in protein synthesis starts, and after 120 min, the translation process has returned to control levels, suggesting that Glu regulates both translation initiation and elongation. Indeed, phosphorylation of the eIF2α is present after 10 min Glu exposure . Glu also regulates the phosphorylation of eukaryotic elongation factor 2 (eEF2) via AMPA and KA receptors at this time frame . Both phosphorylation events (eIF2α and eEF2) are time dependent events, with a kinetics that matches the downregulation of the protein synthesis upon Glu.
The recovery phase of protein synthesis in Glu exposed cells involves an increase in the mechanistic target of rapamycin (mTOR) phosphorylation [17, 90]; this protein is thought to act as a check point that regulates cellular translational capacity since this kinase is capable of transducing extracellular growth factors signals and by these means regulating translation. Once activated (phosphorylated) this kinase favours eukaryotic elongation factor 1A (eEF1A) phosphorylation, needed for translation reinitiation. After 60 min of Glu exposure, an increase of eukaryotic elongation factor 2 kinase (eEF2K) phosphorylation is present . eEF2K phosphorylation is carried out by and by inhibiting its activity and therefore reducing eEF2 phosphorylation levels favouring ribosomal translocation and protein synthesis reinitiation.
6.3. Physiological Consequences of Glutamate-Dependent Protein Synthesis Regulation
Glu biphasic effect in protein synthesis is clear; but what is the physiological importance of this regulation? It is tempting to speculate the downregulation of protein synthesis as a consequence of a massive Glu exposure of glia cells surrounding glutamatergic synapses with the expected metabolic stress of the removal of the neurotransmitter from the synaptic cleft. It is important to mention that Glu concentration can reach a 0.1 mM concentration, well above of the of the glial transporters, which is around 30 μM . Therefore a strict coupling with the Na+/K+-ATPase is present [92, 93]. It is clear then that under periods of sustained synaptic activity glial cells reduce their protein synthesis, in order to restore the Na+ gradient compulsory for neurotransmitter uptake. Besides the Glu uptake, its recycling also consumes energy. Neuronal Glu pools are replenished through the Glu/Gln shuttle .
The reduction of the overall elongation process is frequently linked to the translation of mRNAs with complex structures in their 3′ and 5′ untranslated regions (UTRs). Since translation initiation factors are accumulated and can interact with complex UTRs, in this scenario, Glu also has an important role in the regulation of the translation of specific mRNAs. One of the targets of this type of regulation is the Gln synthetase mRNA, and the interruption of the elongation process favours the translation of Gln synthetized mRNA needed for the referred shuttle .
7. Astrocyte-Neuron Lactate Shuttle
More than twenty years ago new evidences of another role of glial cells emerged, the astrocyte-neuron lactate shuttle (ANLS) . As has been mentioned before, Glu uptake increases Na+ intracellular concentrations leading to the activation of Na+/K+-ATPase forming part of a macromolecular complex. The consumption of ATP activates glycolysis, with the consequent glucose utilization and lactate production. Lactate is released through the action of monocarboxylate transporters (MCT) 1 and 4 that are present in glial cells; once released, lactate is taken up by neurons through MCT 2 and used as an energy substrate. In this scenario glial cells have mainly glycolytic metabolism while neurons display an oxidative metabolism .
Although at the beginning this model raised controversies, genomic and metabolic approaches have shed some light on its importance and it is now well accepted. There are enough evidences of a metabolic compartmentalization between glial cells and neurons; first, Glu treatment in glial cells enhances glucose transport  with an increase in glucose consumption [120–122]. Enzyme lactate dehydrogenase (LDH) isoform 5 that favours lactate production is preferentially expressed in astrocytes, as well as the lactate transporters MCT 1 and 4 that have low affinity to lactate [123, 124], and astrocytes and endothelial cells express glucose transporter 1 (GLUT1). On the other hand, Glu decreases glucose transport in neurons , neurons express preferentially isoform 1 of the LDH that favours the lactate conversion to pyruvate and MCT 2, a transporter with high affinity to lactate , and express glucose transporter 3 (GLUT3) [127, 128].
8. Clinical Implications
It has been documented that loss of glial cells-dependent Glu homeostasis is a prerequisite for excitotoxicity. Glu release from astrocytes has clear pathophysiological implications, ranging from ischemic lesion such as stroke, to white matter injury through demyelinating disorders like multiple sclerosis, to dementias such as Alzheimer’s and Huntington diseases . Although the causative role of aberrant glutamate uptake in these diseases is not always supported by published data, downregulation of GLAST and GLT-1 expression has been correlated with cognitive deficits associated with the diseases mentioned before . Decrease expression and function of GLAST and GLT-1 also correlates with cognitive deficits observed in heavy metal exposure, as lead (Pb) and methylmercury . The reduced GLT-1 expression seen in ALS, schizophrenia, mood and anxiety disorders, Alzheimer’s disease, brain injury, glaucoma, HIV-associated dementia, and addition is regulated at two levels, transcriptional and during mRNA maturation (splicing) ; for example, in ALS an abnormal splicing of Glu transporters mRNA which results in truncated mRNA species has been demonstrated . Furthermore, aberrant Glu transporters expression and function are common to gliomas in order to favour their own growth, invasion, and survival , favouring the notion of protein repertoire regulation in glial cells.
Glial cells sense glutamatergic synaptic activity through Glu receptors and transporters and change their protein repertoire regulating transcription as well as translation of proteins critically involved in their continuous molecular dialogue with neurons. The Glu/Gln and the astrocyte-neuron lactate shuttles are the biochemical signature of coupling and are summarized in Figure 1. Much is left to be learned about the fine regulation of glutamatergic neurotransmission but one thing is for sure that glial cells have an active participation in this process.
Conflict of Interests
The authors declare no competing financial interests.
Zila Martínez-Lozada is supported by SNI-Conacyt and the work in the lab (Arturo Ortega) is supported by Conacyt-México and Fundación Pandea.
- A. Reiner and E. Y. Isacoff, “Tethered ligands reveal glutamate receptor desensitization depends on subunit occupancy,” Nature Chemical Biology, vol. 10, no. 4, pp. 273–280, 2014.
- D.-H. Youn, G. Gerber, and W. A. Sather, “Ionotropic glutamate receptors and voltage-gated Ca2+ channels in long-term potentiation of spinal dorsal horn synapses and pain hypersensitivity,” Neural Plasticity, vol. 2013, Article ID 654257, 19 pages, 2013.
- T. J. Wilding, M. N. Lopez, and J. E. Huettner, “Radial symmetry in a chimeric glutamate receptor pore,” Nature Communications, vol. 5, article 4349, 2014.
- M. Hollmann and S. Heinemann, “Cloned glutamate receptors,” Annual Review of Neuroscience, vol. 17, pp. 31–108, 1994.
- B. Sommer, M. Kohler, R. Sprengel, and P. H. Seeburg, “RNA editing in brain controls a determinant of ion flow in glutamate-gated channels,” Cell, vol. 67, no. 1, pp. 11–19, 1991.
- T. Lopez, A. M. Lopez-Colome, and A. Ortega, “AMPA/KA receptor expression in radial glia,” NeuroReport, vol. 5, no. 4, pp. 504–506, 1994.
- T. Muller, T. Moller, T. Berger, J. Schnitzer, and H. Kettenmann, “Calcium entry through kainate receptors and resulting potassium-channel blockade in Bergmann glial cells,” Science, vol. 256, no. 5063, pp. 1563–16566, 1992.
- B. Herguedas, J. Krieger, and I. H. Greger, “Receptor heteromeric assembly-how it works and why it matters: the case of ionotropic glutamate receptors,” Progress in Molecular Biology and Translational Science, vol. 117, pp. 361–386, 2013.
- S. Kirischuk, H. Kettenmann, and A. Verkhratsky, “Membrane currents and cytoplasmic sodium transients generated by glutamate transport in Bergmann glial cells,” Pflügers Archiv—European Journal of Physiology, vol. 454, no. 2, pp. 245–252, 2007.
- V. Parpura and A. Verkhratsky, “Astroglial amino acid-based transmitter receptors,” Amino Acids, vol. 44, no. 4, pp. 1151–1158, 2013.
- N. Burnashev, A. Khodorova, P. Jonas et al., “Calcium-permeable AMPA-kainate receptors in fusiform cerebellar glial cells,” Science, vol. 256, no. 5063, pp. 1566–1570, 1992.
- M. O. Enkvist, I. Holopainen, and K. E. Akerman, “Glutamate receptor-linked changes in membrane potential and intracellular Ca2+ in primary rat astrocytes,” Glia, vol. 2, no. 6, pp. 397–402, 1989.
- M. Iino, K. Goto, W. Kakegawa et al., “Glia-synapse interaction through Ca2+-permeable AMPA receptors in Bergmann glia,” Science, vol. 292, no. 5518, pp. 926–929, 2001.
- J. T. Porter and K. D. McCarthy, “GFAP-positive hippocampal astrocytes in situ respond to glutamatergic neuroligands with increases in [Ca2+],” GLIA, vol. 13, no. 2, pp. 101–112, 1995.
- I. Barrera, L. C. Hernández-Kelly, F. Castelán, and A. Ortega, “Glutamate-dependent elongation factor-2 phosphorylation in Bergmann glial cells,” Neurochemistry International, vol. 52, no. 6, pp. 1167–1175, 2008.
- E. López-Bayghen, A. Aguirre, and A. Ortega, “Transcriptional regulation through glutamate receptors: involvement of tyrosine kinases,” Journal of Neuroscience Research, vol. 74, no. 5, pp. 717–725, 2003.
- R. C. Zepeda, I. Barrera, F. Castelán et al., “Glutamate-dependent phosphorylation of the mammalian target of rapamycin (mTOR) in Bergmann glial cells,” Neurochemistry International, vol. 55, no. 5, pp. 282–287, 2009.
- S. Ishiuchi, K. Tsuzuki, N. Yamada et al., “Extension of glial processes by activation of Ca2+-permeable AMPA receptor channels,” NeuroReport, vol. 12, no. 4, pp. 745–748, 2001.
- A. Verkhratsky and G. Burnstock, “Purinergic and glutamatergic receptors on astroglia,” in Glutamate and ATP at the Interface of Metabolism and Signaling in the Brain, vol. 11 of Advances in Neurobiology, pp. 55–79, 2014.
- J. R. Vargas, D. K. Takahashi, K. E. Thomson, and K. S. Wilcox, “The expression of kainate receptor subunits in hippocampal astrocytes after experimentally induced status epilepticus,” Journal of Neuropathology and Experimental Neurology, vol. 72, no. 10, pp. 919–932, 2013.
- F. Conti, “Localization of NMDA receptors in the cerebral cortex: a schematic overview,” Brazilian Journal of Medical and Biological Research, vol. 30, no. 5, pp. 555–560, 1997.
- T. López, A. M. López-Colomé, and A. Ortega, “NMDA receptors in cultured radial glia,” FEBS Letters, vol. 405, no. 2, pp. 245–248, 1997.
- C. L. Thompson, D. L. Drewery, H. D. Atkins, F. A. Stephenson, and P. L. Chazot, “Immunohistochemical localization of N-methyl-D-aspartate receptor NR1, NR2A, NR2B and NR2C/D subunits in the adult mammalian cerebellum,” Neuroscience Letters, vol. 283, no. 2, pp. 85–88, 2000.
- O. Palygin, U. Lalo, and Y. Pankratov, “Distinct pharmacological and functional properties of NMDA receptors in mouse cortical astrocytes,” British Journal of Pharmacology, vol. 163, no. 8, pp. 1755–1766, 2011.
- O. Palygin, U. Lalo, A. Verkhratsky, and Y. Pankratov, “Ionotropic NMDA and P2X1/5 receptors mediate synaptically induced Ca2+ signalling in cortical astrocytes,” Cell Calcium, vol. 48, no. 4, pp. 225–231, 2010.
- A. Berthele, S. Platzer, D. J. Laurie et al., “Expression of metabotropic glutamate receptor subtype mRNA (mGluR1-8) in human cerebellum,” NeuroReport, vol. 10, no. 18, pp. 3861–3867, 1999.
- S. Kirischuk, F. Kirchhoff, V. Matyash, H. Kettenmann, and A. Verkhratsky, “Glutamate-triggered calcium signalling in mouse Bergmann glial cells in situ: role of inositol-1,4,5-trisphosphate-mediated intracellular calcium release,” Neuroscience, vol. 92, no. 3, pp. 1051–1059, 1999.
- B. A. Barres, W. J. Koroshetz, K. J. Swartz, L. L. Y. Chun, and D. P. Corey, “Ion channel expression by white matter glia: the O-2A glial progenitor cell,” Neuron, vol. 4, no. 4, pp. 507–524, 1990.
- D. E. Bergles, J. D. B. Roberts, P. Somogyl, and C. E. Jahr, “Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus,” Nature, vol. 405, no. 6783, pp. 187–191, 2000.
- C. L. Bowman, H. K. Kimelberg, M. V. Frangakis, Y. Berwald-Netter, and C. Edwards, “Astrocytes in primary culture have chemically activated sodium channels,” The Journal of Neuroscience, vol. 4, no. 6, pp. 1527–1534, 1984.
- H. Sontheimer, H. Kettenmann, K. H. Backus, and M. Schachner, “Glutamate opens Na+/K+ channels in cultured astrocytes,” Glia, vol. 1, no. 5, pp. 328–336, 1988.
- K. Borges and H. Kettenmann, “Blockade of K+ channels induced by AMPA/kainate receptor activation in mouse oligodendrocyte precursor cells is mediated by Na+ entry,” Journal of Neuroscience Research, vol. 42, no. 4, pp. 579–593, 1995.
- G. Seifert, L. Rehn, M. Weber, and C. Steinhäuser, “AMPA receptor subunits expressed by single astrocytes in the juvenile mouse hippocampus,” Molecular Brain Research, vol. 47, no. 1-2, pp. 286–294, 1997.
- E. Brand-Schieber, S. L. Lowery, and P. Werner, “Select ionotropic glutamate AMPA/kainate receptors are expressed at the astrocyte-vessel interface,” Brain Research, vol. 1007, no. 1-2, pp. 178–182, 2004.
- M. Noda, H. Nakanishi, J. Nabekura, and N. Akaike, “AMPA-kainate subtypes of glutamate receptor in rat cerebral microglia,” The Journal of Neuroscience, vol. 20, no. 1, pp. 251–258, 2000.
- R. Jabs, F. Kirchhoff, H. Kettenmann, and C. Steinhäuser, “Kainate activates Ca2+-permeable glutamate receptors and blocks voltage-gated K+ currents in glial cells of mouse hippocampal slices,” Pflügers Archiv European Journal of Physiology, vol. 426, no. 3-4, pp. 310–319, 1994.
- J. M. García-Barcina, “Expression of kainate-selective glutamate receptor subunits in glial cells of the adult bovine white matter,” The European Journal of Neuroscience, vol. 8, no. 11, pp. 2379–2387, 1996.
- D. G. Puro, J. P. Yuan, and N. J. Sucher, “Activation of NMDA receptor-channels in human retinal Müller glial cells inhibits inward-rectifying potassium currents,” Visual Neuroscience, vol. 13, no. 2, pp. 319–326, 1996.
- F. Conti, S. DeBiasi, A. Minelli, and M. Melone, “Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes,” Glia, vol. 17, no. 3, pp. 254–258, 1996.
- C. G. Schipke, C. Ohlemeyer, M. Matyash, C. Nolte, H. Kettenmann, and F. Kirchhoff, “Astrocytes of the mouse neocortex express functional N-methyl-D-aspartate receptors.,” The FASEB Journal, vol. 15, no. 7, pp. 1270–1272, 2001.
- U. Lalo, Y. Pankratov, F. Kirchhoff, R. A. North, and A. Verkhratsky, “NMDA receptors mediate neuron-to-glia signaling in mouse cortical astrocytes,” The Journal of Neuroscience, vol. 26, no. 10, pp. 2673–2683, 2006.
- J. M. Luque and J. G. Richards, “Expression of NMDA 2B receptor subunit mRNA in bergmann glia,” Glia, vol. 13, no. 3, pp. 228–232, 1995.
- M.-C. Lee, K. K. Ting, S. Adams, B. J. Brew, R. Chung, and G. J. Guillemin, “Characterisation of the expression of NMDA receptors in human astrocytes,” PLoS ONE, vol. 5, no. 11, Article ID e14123, 2010.
- R. Káradóttir, P. Cavelier, L. H. Bergersen, and D. Attwell, “NMDA receptors are expressed in oligodendrocytes and activated in ischaemia,” Nature, vol. 438, no. 7071, pp. 1162–1166, 2005.
- M. G. Salter and R. Fern, “NMDA receptors are expressed in developing oligodendrocyte processes and mediate injury,” Nature, vol. 438, no. 7071, pp. 1167–1171, 2005.
- D. Žiak, A. Chvátal, and E. Syková, “Glutamate-, kainate- and NMDA-evoked membrane currents in identified glial cells in rat spinal cord slice,” Physiological Research, vol. 47, no. 5, pp. 365–375, 1998.
- K. Biber, D. J. Laurie, A. Berthele et al., “Expression and signaling of group I metabotropic glutamate receptors in astrocytes and microglia,” Journal of Neurochemistry, vol. 72, no. 4, pp. 1671–1680, 1999.
- B. Pearce, J. Albrecht, C. Morrow, and S. Murphy, “Astrocyte glutamate receptor activation promotes inositol phospholipid turnover and calcium flux,” Neuroscience Letters, vol. 72, no. 3, pp. 335–340, 1986.
- R. S. Petralia, Y.-X. Wang, A. S. Niedzielski, and R. J. Wenthold, “The metabotropic glutamate receptors, MGLUR2 and MGLUR3, show unique postsynaptic, presynaptic and glial localizations,” Neuroscience, vol. 71, no. 4, pp. 949–976, 1996.
- Y. Tamaru, S. Nomura, N. Mizuno, and R. Shigemoto, “Distribution of metabotropic glutamate receptor mGluR3 in the mouse CNS: differential location relative to pre- and postsynaptic sites,” Neuroscience, vol. 106, no. 3, pp. 481–503, 2001.
- J. J. G. Geurts, G. Wolswijk, L. Bö et al., “Altered expression patterns of group I and II metabotropic glutamate receptors in multiple sclerosis,” Brain, vol. 126, no. 8, pp. 1755–1766, 2003.
- W. Deng, H. Wang, P. A. Rosenberg, J. J. Volpe, and F. E. Jensen, “Role of metabotropic glutamate receptors in oligodendrocyte excitotoxicity and oxidative stress,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 20, pp. 7751–7756, 2004.
- D. L. Taylor, L. T. Diemel, and J. M. Pocock, “Activation of microglial group III metabotropic glutamate receptors protects neurons against microglial neurotoxicity,” The Journal of Neuroscience, vol. 23, no. 6, pp. 2150–2160, 2003.
- D. L. Taylor, F. Jones, E. S. F. Chen Seho Kubota, and J. M. Pocock, “Stimulation of microglial metabotropic glutamate receptor mGlu2 triggers tumor necrosis factor α-induced neurotoxicity in concert with microglial-derived Fas ligand,” The Journal of Neuroscience, vol. 25, no. 11, pp. 2952–2964, 2005.
- P. Shashidharan and A. Plaitakis, “Cloning and characterization of a glutamate transporter cDNA from human cerebellum,” Biochimica et Biophysica Acta, vol. 1216, no. 1, pp. 161–164, 1993.
- K. Watase, K. Hashimoto, M. Kano et al., “Motor discoordination and increased susceptibility to cerebellar injury in GLAST mutant mice,” The European Journal of Neuroscience, vol. 10, no. 3, pp. 976–988, 1998.
- K. P. Lehre and N. C. Danbolt, “The number of glutamate transport subtype molecules at glutamatergic synapses: chemical and stereological quantification in young adult rat brain,” The Journal of Neuroscience, vol. 18, no. 21, pp. 8751–8757, 1998.
- K. P. Lehre, L. M. Levy, O. P. Ottersen, J. Storm-Mathisen, and N. C. Danbolt, “Differential expression of two glial glutamate transporters in the rat brain: quantitative and immunocytochemical observations,” The Journal of Neuroscience, vol. 15, no. 3, part 1, pp. 1835–1853, 1995.
- T. Storck, S. Schulte, K. Hofmann, and W. Stoffel, “Structure, expression, and functional analysis of a Na+-dependent glutamate/aspartate transporter from rat brain,” Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 22, pp. 10955–10959, 1992.
- A. Derouiche and T. Rauen, “Coincidence of L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) immunoreactions in retinal glia: evidence for coupling of GLAST and GS in transmitter clearance,” Journal of Neuroscience Research, vol. 42, no. 1, pp. 131–143, 1995.
- K. P. Lehre, S. Davanger, and N. C. Danbolt, “Localization of the glutamate transporter protein GLAST in rat retina,” Brain Research, vol. 744, no. 1, pp. 129–137, 1997.
- D. V. Pow and N. L. Barnett, “Changing patterns of spatial buffering of glutamate in developing rat retinae are mediated by the Muller cell glutamate transporter GLAST,” Cell and Tissue Research, vol. 297, no. 1, pp. 57–66, 1999.
- V. P. Sarthy, L. Pignataro, T. Pannicke et al., “Glutamate transport by retinal Müller cells in glutamate/aspartate transporter-knockout mice,” Glia, vol. 49, no. 2, pp. 184–196, 2005.
- A. V. Vallat-Decouvelaere, F. Chrétien, G. Gras, G. le Pavec, D. Dormont, and F. Gray, “Expression of excitatory amino acid transporter-1 in brain macrophages and microglia of HIV-infected patients. A neuroprotective role for activated microglia?” Journal of Neuropathology and Experimental Neurology, vol. 62, no. 5, pp. 475–485, 2003.
- W. Chen, C. Aoki, V. Mahadomrongkul et al., “Expression of a variant form of the glutamate transporter GLT1 in neuronal cultures and in neurons and astrocytes in the rat brain,” The Journal of Neuroscience, vol. 22, no. 6, pp. 2142–2152, 2002.
- N. C. Danbolt, J. Storm-Mathisen, and B. I. Kanner, “An [Na+ + K+]coupled L-glutamate transporter purified from rat brain is located in glial cell processes,” Neuroscience, vol. 51, no. 2, pp. 295–310, 1992.
- Ø. Haugeto, K. Ullensvang, L. M. Levy et al., “Brain glutamate transporter proteins form homomultimers,” The Journal of Biological Chemistry, vol. 271, no. 44, pp. 27715–27722, 1996.
- M. Domercq, E. Etxebarria, A. Pérez-Samartín, and C. Matute, “Excitotoxic oligodendrocyte death and axonal damage induced by glutamate transporter inhibition,” Glia, vol. 52, no. 1, pp. 36–46, 2005.
- M. Domercq, M. V. Sanchez-Gomez, P. Areso, and C. Matute, “Expression of glutamate transporters in rat optic nerve oligodendrocytes,” The European Journal of Neuroscience, vol. 11, no. 7, pp. 2226–2236, 1999.
- Z. Martinez-Lozada, C. T. Waggener, K. Kim et al., “Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIβ's actin-binding/-stabilizing domain,” Glia, vol. 62, no. 9, pp. 1543–1558, 2014.
- P. Werner, D. Pitt, and C. S. Raine, “Multiple sclerosis: altered glutamate homeostasis in lesions correlates with oligodendrocyre and axonal damage,” Annals of Neurology, vol. 50, no. 2, pp. 169–180, 2001.
- K. Nakajima, Y. Tohyama, S. Kohsaka, and T. Kurihara, “Ability of rat microglia to uptake extracellular glutamate,” Neuroscience Letters, vol. 307, no. 3, pp. 171–174, 2001.
- F. Conti, S. DeBiasi, A. Minelli, J. D. Rothstein, and M. Melone, “EAAC1, a high-affinity glutamate tranporter, is localized to astrocytes and gabaergic neurons besides pyramidal cells in the rat cerebral cortex,” Cerebral Cortex, vol. 8, no. 2, pp. 108–116, 1998.
- K. Matthias, F. Kirchhoff, G. Seifert et al., “Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus,” The Journal of Neuroscience, vol. 23, no. 5, pp. 1750–1758, 2003.
- V. Montana, Y. Ni, V. Sunjara, X. Hua, and V. Parpura, “Vesicular glutamate transporter-dependent glutamate release from astrocytes,” The Journal of Neuroscience, vol. 24, no. 11, pp. 2633–2642, 2004.
- M. Stenovec, M. Kreft, S. Grilc et al., “Ca2+-dependent mobility of vesicles capturing anti-VGLUT1 antibodies,” Experimental Cell Research, vol. 313, no. 18, pp. 3809–3818, 2007.
- E. Sánchez-Mendoza, M. C. Burguete, M. Castelló-Ruiz et al., “Transient focal cerebral ischemia significantly alters not only EAATs but also VGLUTs expression in rats: relevance of changes in reactive astroglia,” Journal of Neurochemistry, vol. 113, no. 5, pp. 1343–1355, 2010.
- N. C. Danbolt, “Glutamate uptake,” Progress in Neurobiology, vol. 65, no. 1, pp. 1–105, 2001.
- V. Eulenburg and J. Gomeza, “Neurotransmitter transporters expressed in glial cells as regulators of synapse function,” Brain Research Reviews, vol. 63, no. 1-2, pp. 103–112, 2010.
- W.-H. Hu, W. M. Walters, X.-M. Xia, S. A. Karmally, and J. R. Bethea, “Neuronal glutamate transporter EAAT4 is expressed in astrocytes,” Glia, vol. 44, no. 1, pp. 13–25, 2003.
- H. Tang, E. Hornstein, M. Stolovich et al., “Amino acid-induced translation of TOP mRNAs is fully dependent on phosphatidylinositol 3-kinase-mediated signaling, is partially inhibited by rapamycin, and is independent of S6K1 and rpS6 phosphorylation,” Molecular and Cellular Biology, vol. 21, no. 24, pp. 8671–8683, 2001.
- R. P. Shank and G. L. Campbell, “Glutamine, glutamate and other possible regulators of alpha-ketoglutarate and malate uptake by synaptic terminals,” Journal of Neurochemistry, vol. 42, no. 4, pp. 1162–1169, 1984.
- Z. Martínez-Lozada, A. M. Guillem, M. Flores-Méndez et al., “GLAST/EAAT1-induced Glutamine release via SNAT3 in Bergmann glial cells: evidence of a functional and physical coupling,” Journal of Neurochemistry, vol. 125, no. 4, pp. 545–554, 2013.
- W. A. Fairman, R. J. Vandenberg, J. L. Arriza, M. P. Kavanaugh, and S. G. Amara, “An excitatory amino-acid transporter with properties of a ligand-gated chloride channel,” Nature, vol. 375, no. 6532, pp. 599–603, 1995.
- S. Duan, C. M. Anderson, B. A. Stein, and R. A. Swanson, “Glutamate induces rapid upregulation of astrocyte glutamate transport and cell-surface expression of GLAST,” The Journal of Neuroscience, vol. 19, no. 23, pp. 10193–10200, 1999.
- M. I. González and A. Ortega, “Regulation of high-affinity glutamate uptake activity in Bergmann glia cells by glutamate,” Brain Research, vol. 866, no. 1-2, pp. 73–81, 2000.
- C. Murphy-Royal, J. P. Dupuis, J. A. Varela et al., “Surface diffusion of astrocytic glutamate transporters shapes synaptic transmission,” Nature Neuroscience, vol. 18, no. 2, pp. 219–226, 2015.
- K. Abe and H. Saito, “Possible linkage between glutamate transporter and mitogen-activated protein kinase cascade in cultured rat cortical astrocytes,” Journal of Neurochemistry, vol. 76, no. 1, pp. 217–223, 2001.
- A. M. López-Colomé, Z. Martínez-Lozada, A. M. Guillem, E. López, and A. Ortega, “Glutamate transporter-dependent mTOR phosphorylation in Müller glia cells,” ASN Neuro, vol. 4, no. 5, pp. 331–342, 2012.
- Z. Martínez-Lozada, L. C. Hernández-Kelly, J. Aguilera, E. López-Bayghen, and A. Ortega, “Signaling through EAAT-1/GLAST in cultured Bergmann glia cells,” Neurochemistry International, vol. 59, no. 6, pp. 871–879, 2011.
- D. E. Bauer, J. G. Jackson, E. N. Genda, M. M. Montoya, M. Yudkoff, and M. B. Robinson, “The glutamate transporter, GLAST, participates in a macromolecular complex that supports glutamate metabolism,” Neurochemistry International, vol. 61, no. 4, pp. 566–574, 2012.
- M. Gegelashvili, A. Rodriguez-Kern, L. Sung, K. Shimamoto, and G. Gegelashvili, “Glutamate transporter GLAST/EAAT1 directs cell surface expression of FXYD2/gamma subunit of Na, K-ATPase in human fetal astrocytes,” Neurochemistry International, vol. 50, no. 7-8, pp. 916–920, 2007.
- E. M. Rose, J. C. P. Koo, J. E. Antflick, S. M. Ahmed, S. Angers, and D. R. Hampson, “Glutamate transporter coupling to Na,K-ATPase,” The Journal of Neuroscience, vol. 29, no. 25, pp. 8143–8155, 2009.
- K. Cohen-Kashi-Malina, I. Cooper, and V. I. Teichberg, “Mechanisms of glutamate efflux at the blood-brain barrier: involvement of glial cells,” Journal of Cerebral Blood Flow and Metabolism, vol. 32, no. 1, pp. 177–189, 2012.
- V. Parpura, T. A. Basarsky, F. Liu, K. Jeftinija, S. Jeftinija, and P. G. Haydon, “Glutamate-mediated astrocyte-neuron signalling,” Nature, vol. 369, no. 6483, pp. 744–747, 1994.
- S. El Mestikawy, Å. Wallén-Mackenzie, G. M. Fortin, L. Descarries, and L.-E. Trudeau, “From glutamate co-release to vesicular synergy: vesicular glutamate transporters,” Nature Reviews Neuroscience, vol. 12, no. 4, pp. 204–216, 2011.
- S. Takamori, “VGLUTs: ‘exciting’ times for glutamatergic research?” Neuroscience Research, vol. 55, no. 4, pp. 343–351, 2006.
- H. Omote, T. Miyaji, N. Juge, and Y. Moriyama, “Vesicular neurotransmitter transporter: bioenergetics and regulation of glutamate transport,” Biochemistry, vol. 50, no. 25, pp. 5558–5565, 2011.
- Y. Ni and V. Parpura, “Dual regulation of Ca21-dependent glutamate release from astrocytes: Vesicular glutamate transporters and cytosolic glutamate levels,” Glia, vol. 57, no. 12, pp. 1296–1305, 2009.
- S. D. Jeftinija, K. V. Jeftinija, and G. Stefanovic, “Cultured astrocytes express proteins involved in vesicular glutamate release,” Brain Research, vol. 750, no. 1-2, pp. 41–47, 1997.
- R. Zorec, A. Verkhratsky, J. J. Rodríguez, and V. Parpura, “Astrocytic vesicles and gliotransmitters: slowness of vesicular release and synaptobrevin2-laden vesicle nanoarchitecture,” Neuroscience, 2015.
- D. Li, K. Hérault, K. Silm et al., “Lack of evidence for vesicular glutamate transporter expression in mouse astrocytes,” The Journal of Neuroscience, vol. 33, no. 10, pp. 4434–4455, 2013.
- V. Gallo and C. A. Ghiani, “Glutamate receptors in glia: new cells, new inputs and new functions,” Trends in Pharmacological Sciences, vol. 21, no. 7, pp. 252–258, 2000.
- K. J. Mack, S. Kriegler, S. Chang, and S.-Y. Chiu, “Transcription factor expression is induced by axonal stimulation and glutamate in the glia of the developing optic nerve,” Molecular Brain Research, vol. 23, no. 1-2, pp. 73–80, 1994.
- M. Pende, T. L. Fisher, P. B. Simpson, J. T. Russell, J. Blenis, and V. Gallo, “Neurotransmitter- and growth factor-induced cAMP response element binding protein phosphorylation in glial cell progenitors: role of calcium ions, protein kinase C, and mitogen-activated protein kinase/ribosomal S6 kinase pathway,” The Journal of Neuroscience, vol. 17, no. 4, pp. 1291–1301, 1997.
- G. Sanchez and A. Ortega, “AMPA/KA receptor induced AP-1 DNA binding activity in cultured Bergmann glia cells,” NeuroReport, vol. 5, no. 16, pp. 2109–2112, 1994.
- S. Koirala and G. Corfas, “Identification of novel glial genes by single-cell transcriptional profiling of Bergmann glial cells from mouse cerebellum,” PLoS ONE, vol. 5, no. 2, Article ID e9198, 2010.
- E. López-Bayghen and A. Ortega, “Glial glutamate transporters: new actors in brain signaling,” IUBMB Life, vol. 63, no. 10, pp. 816–823, 2011.
- E. López-Bayghen, S. Rosas, F. Castelán, and A. Ortega, “Cerebellar Bergmann glia: an important model to study neuron-glia interactions,” Neuron Glia Biology, vol. 3, no. 2, pp. 155–167, 2007.
- M. E. González-Mejia, M. Morales, L. C. R. Hernández-Kelly, R. C. Zepeda, A. Bernabé, and A. Ortega, “Glutamate-dependent translational regulation in cultured Bergmann glia cells: involvement of p70S6K,” Neuroscience, vol. 141, no. 3, pp. 1389–1398, 2006.
- C. P. Stanners, “Polyribosomes of hamster cells: transit time measurements,” Biophysical Journal, vol. 8, no. 2, pp. 231–251, 1968.
- I. Barrera, M. Flores-Méndez, L. C. Hernández-Kelly et al., “Glutamate regulates eEF1A phosphorylation and ribosomal transit time in Bergmann glial cells,” Neurochemistry International, vol. 57, no. 7, pp. 795–803, 2010.
- X. Wang, W. Li, M. Williams, N. Terada, D. R. Alessi, and C. G. Proud, “Regulation of elongation factor 2 kinase by p90RSK1 and p70 S6 kinase,” The EMBO Journal, vol. 20, no. 16, pp. 4370–4379, 2001.
- N. Sonenberg and A. G. Hinnebusch, “Regulation of translation initiation in eukaryotes: mechanisms and biological targets,” Cell, vol. 136, no. 4, pp. 731–745, 2009.
- M. A. Flores-Méndez, Z. Martínez-Lozada, H. C. Monroy, L. C. Hernández-Kelly, I. Barrera, and A. Ortega, “Glutamate-dependent translational control in cultured bergmann glia cells: EIF2alpha phosphorylation,” Neurochemical Research, vol. 38, no. 7, pp. 1324–1332, 2013.
- L. K. Bak, A. Schousboe, and H. S. Waagepetersen, “The glutamate/GABA-glutamine cycle: aspects of transport, neurotransmitter homeostasis and ammonia transfer,” Journal of Neurochemistry, vol. 98, no. 3, pp. 641–653, 2006.
- D. Shin and C. Park, “N-terminal extension of canine glutamine synthetase created by splicing alters its enzymatic property,” The Journal of Biological Chemistry, vol. 279, no. 2, pp. 1184–1190, 2004.
- L. Pellerin and P. J. Magistretti, “Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 22, pp. 10625–10629, 1994.
- L. Pellerin, A.-K. Bouzier-Sore, A. Aubert et al., “Activity-dependent regulation of energy metabolism by astrocytes: an update,” Glia, vol. 55, no. 12, pp. 1251–1262, 2007.
- A. K. Bouzier-Sore, P. Voisin, V. Bouchaud, E. Bezancon, J.-M. Franconi, and L. Pellerin, “Competition between glucose and lactate as oxidative energy substrates in both neurons and astrocytes: a comparative NMR study,” The European Journal of Neuroscience, vol. 24, no. 6, pp. 1687–1694, 2006.
- A. Loaiza, O. H. Porras, and L. F. Barros, “Glutamate triggers rapid glucose transport stimulation in astrocytes as evidenced by real-time confocal microscopy,” The Journal of Neuroscience, vol. 23, no. 19, pp. 7337–7342, 2003.
- H. S. Waagepetersen, I. J. Bakken, O. M. Larsson, U. Sonnewald, and A. Schousboe, “Comparison of lactate and glucose metabolism in cultured neocortical neurons and astrocytes using 13C-NMR spectroscopy,” Developmental Neuroscience, vol. 20, no. 4-5, pp. 310–320, 1998.
- D. Z. Gerhart, B. E. Enerson, O. Y. Zhdankina, R. L. Leino, and L. R. Drewes, “Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats,” The American Journal of Physiology—Endocrinology and Metabolism, vol. 273, no. 1, pp. E207–E213, 1997.
- A. Rafiki, J. L. Boulland, A. P. Halestrap, O. P. Ottersen, and L. Bergersen, “Highly differential expression of the monocarboxylate transporters MCT2 and MCT4 in the developing rat brain,” Neuroscience, vol. 122, no. 3, pp. 677–688, 2003.
- O. H. Porras, A. Loaiza, and L. Felipe Barros, “Glutamate mediates acute glucose transport inhibition in hippocampal neurons,” The Journal of Neuroscience, vol. 24, no. 43, pp. 9669–9673, 2004.
- K. Pierre, P. J. Magistretti, and L. Pellerin, “MCT2 is a major neuronal monocarboxylate transporter in the adult mouse brain,” Journal of Cerebral Blood Flow and Metabolism, vol. 22, no. 5, pp. 586–595, 2002.
- F. Maher, “Immunolocalization of GLUT1 and GLUT3 glucose transporters in primary cultured neurons and glia,” Journal of Neuroscience Research, vol. 42, no. 4, pp. 459–469, 1995.
- E. E. Benarroch, “Brain glucose transporters: implications for neurologic disease,” Neurology, vol. 82, no. 15, pp. 1374–1379, 2014.
- A. M. D. J. Domingues, M. Taylor, and R. Fern, “Glia as transmitter sources and sensors in health and disease,” Neurochemistry International, vol. 57, no. 4, pp. 359–366, 2010.
- G. Gegelashvili and O. J. Bjerrum, “High-affinity glutamate transporters in chronic pain: an emerging therapeutic target,” The Journal of Neurochemistry, vol. 131, no. 6, pp. 712–730, 2014.
- P. Karki, K. Smith, J. Johnson Jr., M. Aschner, and E. Lee, “Role of transcription factor yin yang 1 in manganese-induced reduction of astrocytic glutamate transporters: putative mechanism for manganese-induced neurotoxicity,” Neurochemistry International, 2014.
- T. L. Lauriat and L. A. McInnes, “EAAT2 regulation and splicing: relevance to psychiatric and neurological disorders,” Molecular Psychiatry, vol. 12, no. 12, pp. 1065–1078, 2007.
- C. L. G. Lin, L. A. Bristol, L. Jin et al., “Aberrant RNA processing in a neurodegenerative disease: the cause for absent EAAT2, a glutamate transporter, in amyotrophic lateral sclerosis,” Neuron, vol. 20, no. 3, pp. 589–602, 1998.
- S. M. Robert and H. Sontheimer, “Glutamate transporters in the biology of malignant gliomas,” Cellular and Molecular Life Sciences, vol. 71, no. 10, pp. 1839–1854, 2014.
Copyright © 2015 Zila Martínez-Lozada and Arturo Ortega. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.