Generation of New Neurons in Dorsal Root Ganglia in Adult Rats after Peripheral Nerve Crush Injury

Figure 7

Immunofluorescence images of DRG after crush lesion and BrdU treatment for the qualitative evaluation. 15 days after crush lesion, many small cell nuclei surrounding ganglionic neurons as well as some nuclei belonging to sensory neurons are found to be BrdU-positive (a, arrows). Cells localized surrounding the ganglionic neurons are immunolabeled with Ki67, an endogenous marker for proliferation 3 days after crush lesion (b). A double labeling using anti-Ki67 (green) associated with anti-peripherin (red), 7 days after injury, shows a coexpression of the two markers in ganglionic neurons (c). Scale bars: a–c = 20 µm.