Chondroitin 6-Sulfation Regulates Perineuronal Net Formation by Controlling the Stability of Aggrecan

Figure 4

Developmental formation and morphological features of WFA-positive and CS56-positive PNNs. (a) Formation of WFA-positive and CS56-positive PNNs in the cerebral cortex of C6ST-1 TG mice during development. Note that CS56-positive PNNs were not colocalized with conventional WFA-positive PNNs. The numbers of both WFA-positive (b) and CS56-positive PNNs (c) increased until P60 and were maintained in aged C6ST-1 TG mice. Error bars represent SEM; -3. (d) Three-dimensional reconstruction revealed a distinct lattice-like structure of WFA-positive PNNs in both 6-month-old adult WT and C6ST-1 TG mice. In contrast, CS56-positive PNNs in C6ST-1 mice were sparse dot-like structures. Right: magnification of boxed regions in the left panels. VGluT1-labeled presynaptic terminals (arrowheads) were embedded in the meshwork of WFA-positive PNNs, whereas these terminals were not tightly surrounded by CS56-positive PNNs in C6ST-1 mice. Scale bars represent 50 μm (a), 5 μm ((d), left panels), and 1 μm ((d), right panels).

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