Neural Plasticity

Research Article

Chronic Mild Stress Modulates Activity-Dependent Transcription of BDNF in Rat Hippocampal Slices

Table 1

Antibodies conditions used in the Western blot analyses.
GenePrimary antibodySecondary antibody
Phospho-ERK1/2 Y204/Y187
(42–44 kDa)		1 : 1000 (Cell Signaling; #4370)
4°C, O/N		Anti-mouse,
1 : 2000, RT, 1 h
ERK1/2
(42–44 kDa)		1 : 5000 (Sigma; M3807),
RT, 2 h		Anti-rabbit,
1 : 5000, RT, 1 h
Phospho-CREB S133
(43 kDa)		1 : 1000 (Cell Signaling; #4276),
4°C, O/N		Anti-rabbit,
1 : 2000, RT, 1 h
CREB
(43 kDa)		1 : 1000 (Cell Signaling; #9197),
4°C, O/N		Anti-rabbit,
1 : 2000, RT, 1 h
Phospho-AKT S473
(60 kDa)		1 : 1000 (Cell Signaling; #4060),
4°C, O/N		Anti-rabbit,
1 : 2000, RT, 1 h
AKT
(60 kDa)		1 : 1000 (Cell Signaling; #9272),
RT, 2 h		Anti-rabbit,
1 : 1000, RT, 1 h
Phospho-GSK3β S9
(46 kDa)		1 : 1000 (Cell Signaling; #9336),
4°C, O/N		Anti-rabbit,
1 : 5000, RT, 1 h
GSK3β
(46 kDa)		1 : 2500 (BD Transduction; 610201)
RT, 2 h		Anti-mouse,
1 : 5000, RT, 1 h
β-ACTIN1 : 10000 (Sigma; A5441),
RT, 1 h		Anti-mouse,
1 : 10000, RT, 1 h