Reorganization of Synaptic Connections and Perineuronal Nets in the Deep Cerebellar Nuclei of Purkinje Cell Degeneration Mutant Mice

Table 1

Antibodies and markers.

Marker	Detected Component	Source	Dilution		Reference
			IHC	WB
PN-constituents
Human antiaggrecan (HAG, clone 7D4)	N-terminal aggrecan, core protein (AGG)	Serotec	1 : 10		[61]
Antiaggrecan (AB1031)	Amino acids 1177–1326 of mouse aggrecan	Millipore	1 : 200		[63]
Antibrevican (B50)	50 kDa cleavage fragment of brevican, brain enriched hyaluronan-binding protein	Dr. R. Matthews	1 : 2000	1 : 2000	[64]
Antibrevican (756)	Mainly 90 kDa cleavage fragment of brevican, brain enriched hyaluronan-binding protein	Dr. R. Matthews	1 : 1000
Antibrevican (610894)	80 kDa N-terminal fragment and full length up to 145 kDa	BD Bioscience	1 : 1000	1 : 1250	[65]
Biotinylated hyaluronic Acid Binding Protein (bHABP)	Hyaluronan	Cape Cod	1 : 100		[66]
Antihyaluronan and proteoglycan link protein 1 (HAPLN1/Crtl-1)	NS0-derived rhHAPLN1	R&D Systems	1 : 400	1 : 1000	[67]
Antihyaluronan and proteoglycan link protein 4 (HAPLN4)	NS0-derived recombinant human HAPLN4. Gln30-Val402	R&D Systems	1 : 500	1 : 1000
anti-tenascin-R (clone 619)	Protein backbone of tenascin-R	R&D Systems	1 : 100		[67]
Glial marker
Glial fibrillary acid protein (GFAP)	a 50 kDa intracytoplasmic filamentous protein in astrocytes	Dako	1 : 1000	1 : 3000	[68]
Transmitter
Antivesicular glutamate transporter 1 (vGlut1, #135304)	Glutamate transporter 1 in the membrane of synaptic vesicles	Synaptic Systems	1 : 500	1 : 5000	[69]
Antivesicular glutamate transporter 2 (vGlut2, #135404)	Glutamate transporter 2 in the membrane of synaptic vesicles	Synaptic Systems	1 : 500		[69]
GAD65/67	C-terminal region of human GAD 65 and GAD 67	Sigma	1 : 5000	1 : 15000	[61]
Calcium binding Proteins
Anticalbindin (AB1778)	Recombinant calbindin	Millipore	1 : 1000		[70]
Cytoskeletal proteins
Anti-β-actin	β-cytoplasmic actin N-terminal peptide	Sigma-Aldrich		1 : 10000	[71]