Increases in Retrograde Injury Signaling Complex-Related Transcripts in Central Axons following Injury

Figure 2

Axotomy results in biphasic axonal increase of RIS-associated transcript levels. Real-time RT-PCR was used to quantify levels of putative RIS-associated mRNAs and -actin in axons after axotomy. All values are displayed relative to uninjured controls. Significant increases in importin 1, RanBP1, and -actin mRNA levels at 1 hour after axotomy, suppression at 6 and 15 hrs after axotomy, and increases again at 24 hours after axotomy for importin 1 and RanBP1 were observed. No significant changes were observed in vimentin transcript levels. Error bars represent standard deviations. indicates significant difference from control (p < 0.05) based on pairwise fixed allocation test. for 1 and 24 hrs, and for 6 and 15 hrs.